中文摘要：

目的观察静压力对血管平滑肌细胞（vascular smooth muscle cells,VSMCs）荷脂情况的影响。方法原代培养大鼠主动脉血管平滑肌细胞,取第4~10代与ox-LDL50μg/mL共孵育,用自行研制的压力可调细胞培养箱以不同压力加压处理（0 kPa、8 kPa、16 kPa和24 kPa）。HPLC法观察静压力对VSMCs荷脂的影响;用免疫印迹法检测小凹蛋白1（caveolin-1）的表达与磷酸化情况;用下调caveolin-1活性的药物干预后再用HPLC法观察血管平滑肌细胞胆固醇含量。结果静压力可以减少ox-LDL诱导的VSMCs内胆固醇含量,上调caveo-lin-1的磷酸化;其中以16 kPa压力时作用最明显。SB203580可显著抑制caveolin-1磷酸化,从而导致16 kPa静压力下VSMCs内胆固醇含量增加。结论静压力可通过调节caveolin-1磷酸化抑制ox-LDL诱导的VSMCs胆固醇蓄积。

英文摘要：

Objective To investigate the effect and the mechanism of hydrostatic pressure on the cholesterol accumulation in vascular smooth muscle cells（VSMCs）. Methods VSMCs from rat aorta were incubated with 50 μg/mL ox-LDL,at the same time,treated by different pressures of 0 kPa,8 kPa,16 kPa and 24 kPa in a self-manufactured pressure-adjustable cell incubator for 24hrs respectively.The high performance liquid chromatograph（HPLC） was used to detect the concentration of total cholesterol and free cholesterol.Western-blot was used to analyze the expression and the phosphorylation of caveolin-1.Results Hydrostatic pressure significantly accelerated the cholesterol accumulation in VSMCs compared with normal pressure.At the same time,the phosphorylation of caveolin-1 was significantly increased by hydrostatic pressures,with the peak in 16 kPa.SB203580 was used to inhibit caveolin-1 phosphorylation which could impaire the descending effect of hydrostatic pressure on cholesterol accumulation in VSMCs.Conclusion Hydrostatic pressure inhibits cholesterol accumulation in VSMCs possibly through the caveolin-1.