位置:成果数据库 > 期刊 > 期刊详情页
牛磺酸对锰染毒大鼠纹状体神经组织细胞钙平衡调节的影响
  • ISSN号:1001-9391
  • 期刊名称:《中华劳动卫生职业病杂志》
  • 时间:0
  • 分类:R135.1[医药卫生—劳动卫生;医药卫生—公共卫生与预防医学]
  • 作者机构:[1]厦门医学院预防医学教研室,361023, [2]山西省中医院, [3]广西医科大学公共卫生学院
  • 相关基金:国家自然科学基金资助项目(30260095)
中文摘要:

目的 观察锰和活性钙调素含量及Na^+-K^+-ATPase、Ca^2+-Mg^2+-ATPase活力变化,探讨牛磺酸对锰染毒大鼠神经毒性的影响.方法 取SPF级SD健康雄性大鼠156只,随机分为对照组,10、15、20 mg/kg 3个剂量的染锰组和9个牛磺酸干预组,牛横酸干预剂量分别为100、150、200 mg/kg,每组12只大鼠.干预12周后,分离出大鼠纹状体,分别进行锰和活性钙调素含量及Na^+-K^+-ATPase、Ca^2+-Mg^2+-ATPase活力的测定.结果 各染锰组和牛磺酸干预组纹状体锰含量均高于对照组,差异均有统计学意义(均P〈0.05);10 mg/kg染锰组活性钙调素含量高于对照组(P〈0.05),150、200 mg/kg的牛磺酸干预明显降低10 mg/kg锰染毒大鼠纹状体活性钙调素含量,差异均有统计学意义(均P〈0.05);染锰组大鼠纹状体Na^+-K^+-ATPase、Ca^2+-Mg^2+-ATPase活力水平均低于对照组(均P〈0.05),150 mg/kg牛磺酸干预明显增加10 mg/kg锰染毒大鼠纹状体Na^+-K^+-ATPase活力水平(P〈0.05),150 mg/kg牛磺酸干预明显增加15 mg/kg锰染毒大鼠纹状体Ca^2+-Mg^2+-ATPase活力水平(P〈0.05),200 mg/kg牛磺酸干预明显增加10 mg/kg锰染毒大鼠纹状体Ca^2+-Mg^2+-ATPase活力水平(P〈0.05).结论 锰可降低大鼠纹状体中Na^+-K^+-ATPase、Ca^2+-Mg^2+-ATPase活力,低剂量锰可致活性钙调素含量升高;牛磺酸在一定程度上可对抗锰所致的Na^+-K^+-ATPase、Ca^2+-Mg^2+-ATPase活力降低作用,对锰所致的活性钙调素异常变化发挥调节作用.

英文摘要:

Objective To elucidate the effect of taurine on neurotoxicity induced by Mn by investigating activities of Na^+-K^+-ATPase and Ca^2+-Mg^2+-ATPase and content of Mn and active calmodulin in manganese exposed rats. Methods 156 male SD rats were randomly divided into 1 control group, 3 manganese exposed groups (10, 15, and 20 mg/kg respectively), and 9 taurine intervened groups based on orthogonal design (doses of taurine intervention were 100,150, and 200 mg/kg respectively), with 12 rats in each group. After 12 weeks of exposure, all rats were decapitated and corpus striatums were removed, activities of Na^+-K^+-ATPase and Ca^2+-Mg^2+-ATPase and content of Mn and active calmodulin were analyzed. Results The corpus striatum Mn content of the 3 dose groups exposed to Mn and 9 taurine intervened groups were significantly higher than that of the control group (P〈0.05). Active calmodulin content in 10 mg/kg manganese exposed group was significantly higher than that of the control group (P〈0.05). 150 and 200 mg/kg of taurine could decrease active calmodulin content of the group exposed to 10 mg/kg of Mn (P〈0.05). The corpus striatum activities of Na^+-K^+-ATPase and Ca^2+-Mg^2+-ATPase of the 3 dose groups exposed to Mn were significantly lower than that of the control group (P〈0.05). 150 mg/kg of taurine could increase activities of Na^+-K^+-ATPase of the group exposed to 10 mg/kg of Mn(P〈0.05). 150 and 200 mg/kg of taurine could respectively improve activities of Ca^2+-Mg^2+-ATPase of the group exposed to 15,10 mg/kg of Mn (P〈0.05). Conclusion Mn can decrease the rats corpus striatum activities of Na^+-K^+-ATPase and Ca^2+-Mg^2+-ATPase, effect level of active calmodulin in relation to dose of Mn, to a certain extent, taurine could regulate activities of Na^+-K^+-ATPase and Ca^2+-Mg^2+-ATPase and improve the level of active calmodulin.

同期刊论文项目
同项目期刊论文
期刊信息
  • 《中华劳动卫生职业病杂志》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中华医学会
  • 主编:
  • 地址:天津河东区华越道6号
  • 邮编:300011
  • 邮箱:cjoh1983@163.com
  • 电话:022-24333581
  • 国际标准刊号:ISSN:1001-9391
  • 国内统一刊号:ISSN:12-1094/R
  • 邮发代号:6-50
  • 获奖情况:
  • 2000年中华医学会优秀期刊银奖,2000年天津市优秀期刊奖,2001中华预防医学会优秀期刊一等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),波兰哥白尼索引,荷兰文摘与引文数据库,美国生物医学检索系统,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2000版)
  • 被引量:16727