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中文摘要：
本研究利用PCR方法扩增番鸭细小病毒（Muscovy duck parvovirus,MPV）非结构蛋白NS1基因,并将其克隆至原核表达载体pET32a（＋）,构建重组表达质粒pET32a-NS1。将其转化受体菌E.coli Rosetta感受态细胞,经IPTG诱导表达。SDS-PAGE和Western blot试验结果表明,NS1基因在大肠杆菌中得到了表达,并且可与抗MPV的多克隆抗体发生特异性反应。MPV NS1基因的成功表达为研制MPV的诊断试剂盒、基因工程疫苗等奠定了基础。
英文摘要：
The NS1 gene of Muscovy duck parvovirus（MPV） was amplified by PCR and inserted into multiple clone sites of pET32a（＋） vector.The insertion of NS1 gene was confirmed in restriction endonuclease analysis and sequencing.The recombinant plasmid was transformed into E.coli Rosetta and NS1protein was expressed with induction of 1.0 mmol/ L IPTG.The recombinant NS1 protein was visualized in SDS-PAGE and recognized by MPV specific antibody in Western blot.The successful expression of NS1 gene laid the foundation for development of diagnosis reagent kit and genetically engineering vaccine.
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