傅里叶显微红外光谱成像技术可收集微小物体的红外光谱信息,针对组织切片和细胞进行扫描,记录选择区域像素位点的红外光谱信息,并通过计算机软件构建出组织和细胞中光谱吸收峰的二维和三维光谱映射图像。由于红外光谱对生物大分子的敏感性,通过该技术可以有效分析组织和细胞的生化构成、分子分布、代谢变化。本文阐述了傅里叶显微红外光谱成像技术原理,综述了该技术在生物学、病理学、临床医学中的研究进展,并探讨其在法医学中的潜在应用价值。
Fourier transformation infrared microspectroscopy(FTIR-MSP) mapping technique can collect the infrared information from micro-samples and scan the tissue slides and cells.The infrared spectral information of pixels from the collected regions is recorded and infrared spectral maps are constructed by computer software.The 2D and 3D mapping images are reflected based on the distributions of absorbance bands.The biochemical compositions,molecular distribution,metabolic changes of tissues and cells are analyzed by the technique due to infrared spectroscopy being sensitive to biomolecules.The article reviews the recent research of FTIR-MSP mapping and explores the future potential value in forensic science practice.