中文摘要：

目的 探讨切应力对与血管平滑肌细胞（VSMCs）联合培养的内皮细胞（ECs）中微管的聚集重构的影响，为阐明应力诱导血管重建的分子机制提供一些实验证据。方法应用ECs与VSMCs联合培养的平行平板流动腔系统，给ECs面施加15dyne／cm^2的层流切应力，以静态条件下联合培养的ECs为对照组，用Western Blot、免疫荧光细胞化学和图像分析等技术，研究切应力作用下与VSMCs联合培养的ECs的微管聚集的变化。结果静态联合培养组，ECs微管骨架的排列是稀疏、发散和无规律的。切应力诱导了ECs的微管的重构，微管骨架变得有序，朝切应力的方向规律的排列。切应力能够促进ECs的微管聚集，与对照组相比，切应力作用下的ECs内多聚微管的数量增加，切应力作用3h，ECs内多聚微管的数量达到峰值，之后开始下降。结论切应力诱导和促进了EC的微管骨架发生重构（聚集）。结果提示：微管可能是机械应力刺激作用的靶标，应力可能通过它改变ECs的形态，影响细胞的黏附与迁移等功能．

英文摘要：

Objective The influence of shear stress on the assembly of microtubules in endothelial cells （ECs） was elucidated in the co-culture system with vascular smooth muscle cells to provide some experimental evidences for molecular mechanisms of shear stress-induced vascular remodeling, Methods The assembly of microtubules in ECs co-cultured with vascular smooth muscle cells （VSMCs） under shear stressor 15dyne/cm^2- was examined by Western Blot, immunocytochemistry and image analysis. ECs were co-cultured with VSMCs under static conditions as a control. Results The microtubule arrangements in the control group showed sparsely, emanative and ruleless, After laminar shear stress （1.5Pa, 15dynes/cm^2） applied to ECs co-cultured with VSMCs for 12h, a distinct change in microtubule arrangement appeared to the regular state and oriented to the direction of shear stress, Application of shear stress to the co-cultured ECs also promoted the quantitative assembly of microtubules in ECs. The polymeric tubulin mass became decreased in ECs after exposed to shear stress for 3 hours. Conclusions Shear stress induces and promotes microtubule assembly in the co-cultured ECs. Furthermore, these data suggest that microtubules could be a potential target for translating changes in externally applied mechanical stimulation to alterations in cellular phenotype and functions such as adhesion and migration.