利用SSR分子标记技术构建10个木薯商业品种的指纹图谱。通过基因组多态性分析,从已定位于木薯连锁群的44对SSR引物中筛选到22对扩增效果好的引物,经统计分析发现,用其中4对引物ssry-13、ssry-19、ssry-23和ssry-45的组合能够建立该组木薯商业品种的指纹图谱,品种鉴定的置信概率达到99.9985%。
SSR ( simple sequence repeat) markers were used to find reliable fingerprint to identify 10 commercial cassava cultivars in this study. A total of 22 pair primers being polymorphic and informative selected out of 44 SSR markers localized in cassava genetic linkages. By genome screening and data analysis, an acceptable DNA fingerprint was constructed with 4 pair SSR primers, ssry-13, ssry-19, ssry-23 and ssry-45, that could be used to identify these commercial cassava cultivars with 99. 9985% of probability.