东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

海昆肾喜对糖尿病肾病大鼠肾脏保护作用及其对肾组织CTGF蛋白表达的影响

ISSN号：1673-1727
期刊名称：《中华中医药杂志》
时间：0
分类：R587.2[医药卫生—内分泌;医药卫生—临床医学;医药卫生—内科学] R692[医药卫生—泌尿科学;医药卫生—临床医学;医药卫生—外科学]
作者机构：[1]北京中医药大学,北京100029
相关基金：教育部资助项目（No.IRT0413）,国家自然科学基金重点资助项目（No.30130220）,高等学校学科创新引智计划资助项目（No.B07007）

关键词：糖尿病肾病, 海昆肾喜, 链脲佐菌素, 结缔组织生长因子, 大鼠, Diabetic nephropathy, HaikunShenxi, Streptozotocin, CTGF

中文摘要：

目的：探讨海昆肾喜对糖尿病肾病大鼠肾组织结缔组织生长因子（CTGF）蛋白表达的影响。方法：60只大鼠随机分为2组,正常组10只,造模组50只。造模组采用空腹一次性腹腔注射链脲佐菌素造成糖尿病模型,模型组大鼠按55mg/kg,腹腔一次性注射链脲佐菌素,72h后血糖≥16.7mmol/L,尿糖≥4个＋者为糖尿病造模成功。将造模成功的大鼠按血糖高低随机分组,分为模型组、厄贝沙坦组、海昆小剂量组和海昆大剂量组,给药12周。第4、8、12周末收集大鼠24小时尿量及检测尿蛋白量。12周末处死大鼠,取血并分离血清检测血清肌酐（Scr）、尿素氮（BUN）和甘油三酯（TG）等,采用HE、Mallory染色观察肾组织病理改变,并应用免疫组化检测肾组织CTGF蛋白的表达。结果：海昆肾喜能够降低糖尿病大鼠的蛋白尿,增加体质量,降低Scr和BUN,改善肾功能,调节TG,改善脂代谢紊乱,海昆肾喜能够明显减轻糖尿病肾病大鼠肾组织病理损害,并且能够降低糖尿病大鼠肾小管上皮细胞、肾间质细胞内的CTGF在细胞浆内的表达（P〈0.01）。结论：海昆肾喜可能抑制CTGF蛋白的过度表达,进而减缓糖尿病肾病的病程进展。

英文摘要：

Objective： To study the effect of HaikunShenxi on expression of connective tissue growth factor（CTGF） in renal tubules of STZ-induced diabetic nephropathy rats.Methods： SD rats were randomly divided into two groups： normal control and diabetic nephropathy.Diabetes was induced by intraperitoneal injection of STZ.The diabetic models were affirmed upon blood glucose ≥16.5 mmol/L,urinary glucose ≥ ＋＋＋＋ 72h after the injection.And the diabetic rats were randomly divided into four groups： model group,irbesartan treatment group,low and high dose HaikunShenxi treatment group.24-hour urine volume,24-hour urinary protein excretion were examined at week 4,8,and 12 respectively.All the rats were sacrificed at week 12;body weight,kidney weight,and serum excretion,serum creatinine,blood urea nitroge were detected.Kidney were taken for pathological examination（HE,Mallory stain）,The expressions of CTGF in rat renal were detected respectively by immunohistochemistry and in situ hybridization.Result： 24-hour urine volume,24-hour urinary protein excretion were examined at week 4,8,and 12 respectively.All the rats were sacrificed at week 12;body weight,kidney weight,and serum excretion,serum creatinine,blood urea nitroge was improved by HaikunShenxi preparation.Haikunshenxi can alleviate severe pathological changes of kidney structure in rats with DN,The expression of CTGF in the HaikunShenxi treatment group was depressed greatly.Conclusions： The overexpression of CTGF in renal tissue of diabetic rats may be one of the mechanisms of diabetic nephropathy,haikunshenxi had certainly protective effects on it.

同期刊论文项目