为克服传统的种子活力测定方法存在的测量时间长、种子用量大、测量准确性差和灵敏度低等缺欠,提出了一种以89C55单片机为核心实现对种子活力的快速检测系统.该系统通过测量种子吸胀初期其化学发光(CL)探针试剂介导的CL强度来表征种子的活力.系统基于超弱发光探测技术,采用单光子计数模块(SPCM)来接收CL,具有很高的精度和信噪比,自带步进电机驱动和温度控制,通过通用串行总线(USB)接口与上位机连接,实时分析处理数据并显示.实验结果表明,该系统运行稳定,具有低成本、高精度和方便实用等优点.
In order to solve the disadvantages of the traditional methods for measuring seed vigor, such as long measuring time ,massive consumption of seed,low measuring accuracy, low sensitivity and so on,a rapid optical measurement system for seed vigor is proposed,which takes 89C55 single-chip microcomputer as control center. By measuring the chemiluminescence (CL) intensity of seed to evaluate seed vigor. The CL is tested by using single Photon counting module(SPCM) with high sensitivity and signal-to-noise ratio based on ultra-weak luminescence detection technique. The system is driven by step motor. The results show that the system can work stably,and has lots of values such as low cost,high precision and convenience.