在含有1 mmol/L磷酸的培养基中设置不同浓度的钙离子、镉离子和钙离子通道抑制剂Verpam il,对根内球囊霉Glomus intraradices孢子萌发、菌丝极性生长以及菌丝分支进行了研究.结果表明:钙离子促进了孢子萌发、菌丝极性生长和菌丝分支;镉离子起到了强烈的抑制作用;然而钙离子没有减弱重金属对孢子萌发和菌丝生长的抑制;与此同时钙离子通道阻断剂抑制了菌丝生长和分支,并没有减弱重金属的毒害作用,镉离子并不是主要通过钙离子通道进入AMF的菌丝内.
The influence of CaCl2,CdCl2 and inhibitor Verpamil of calcium channel on the spore germination,hyphal growth and hyphal branching was studied in the medium contained 1 mmol/L phosphoric acid.The results suggested that spore germination,hyphal growth and hyphal branching were enhanced by Calcium ion and inhibited by Cadmium ion.However,Calcium didn't decrease the toxicity of Cadmium to spore germination and hyphal growth.Hyphal growth and hyphal branching were inhibited by Calcium channel inhibitor Verpamil.The toxicity of heavy metal was not decreased by Verpamil.It also showed that Cadmium transported into hyphal interior was not mainly mediated by Calcium channels.