中文摘要：

从羊膜的液体孤立的干细胞作为房间治疗和织物工程的一个有希望的候选人被显示出。然而， maxillofacial 骨头织物工程的地里的羊膜的导出液体的干细胞(AFSC ) 的试验性、现出症状之前的潜的应用仍然是有限的。在这研究，老鼠 AFSC 成功地在 vitro 被收获并且描绘。老鼠 AFSC 显示出典型 fibroblastoid 形态学，稳定的增长活动和多区别潜力。Flow-cytometry 分析为象 CD34 和 CD45 那样的造血的标记证明这些房间为 CD29， CD44，和 CD90 是积极的，当否定时。与血小板富人一起的 AFSCs-premixed 的再生表演在牙槽的骨头缺点的恢复的血浆(PRP ) 胶化进一步用一只修改老鼠被调查颚的牙槽的缺点模型。微型电脑断层摄影术和组织学的检查在操作与控制组作比较以后在 4 和 8 个星期与 PRP 胶化显示出 AFSCs-premixed 的一个优异再生能力。而且，植入的 AFSC 能在缺点地点幸存并且直接参予骨头织物新生。一起拿，这些结果为牙槽的缺点恢复显示了基于 AFSCs 的牙槽的骨头织物工程策略的可行性。

英文摘要：

Stem cells isolated from the amniotic fluid have been shown as a promising candidate for cell therapy and tissue engineering. However, the experimental and preclinical applications of amni- otic fluid-derived stem cells （AFSCs） in the very field of maxillofacial bone tissue engineering are still limited. In this study, rat AFSCs were successfully harvested and characterized in vitro. The rat AFSCs showed typical fibroblastoid morphology, stable proliferation activity and multi- differentiation potential. Flow-cytometry analysis demonstrated that these cells were positive for CD29, CD44, and CD90, while negative for hematopoietic markers such as CD34 and CD45. The regenerative performance of AFSCs-premixed with platelet rich plasma （PRP） gel in restoration of alveolar bone defect was further investigated using a modified rat maxillary alveolar defect model. Micro-computer tomography and histological examination showed a superior regenerative cap- acity of AFSCs-premixed with PRP gel at both 4 and 8 weeks after operation comparing with con- trol groups. Moreover, the implanted AFSCs can survive in the defect site and directly participate in the bone tissue regeneration. Taken together, these results indicated the feasibility of an AFSCs-based alveolar bone tissue engineering strategy for alveolar defect restoration.