中文摘要：

以便测定在 vitro 的牛的免疫不全病毒(BIV ) 感染，一根 BIV 指示物房间线(BIVL ) 被 transfecting 婴儿仓鼠肾房间与包含一个 BIV 长终端重复倡导者驾驶的萤火虫酶基因的记者 plasmids 建立。BIV 激活 LTR 的倡导者活动在感染之上表示酶。BIV 感染能由因此由酶活动的察觉确定了。比作过去常检测 BIV 感染的标准试金，基于 BIVL 的试金是比更敏感的 10 次基于用户终端设备的试金，并且与病毒的衣壳蛋白质有类似的敏感西方的污点试金。BIV 指示物房间线能明确地检测 BIV 感染。感染的 BIVL 房间显示出的 BIV 的酶活动一倍依赖的举止，和 60 h 张贴感染是最佳的时间检测 BIV 感染。BIVL 房间的酶活动与 BIV 衣壳蛋白质表示相关。而且，一种线性关系在 MOI 和酶表示的激活的紧张之间被发现。简言之， BIV 指示物房间线是一容易，柔韧并且为监视 BIV 感染的 quantitive 方法。关键词牛的免疫不全病毒(BIV )- 牛的起泡沫的病毒(BFV )- 酶 - 指示物房间线基础项目：为应用基本研究(08JCZDJC21000 ) 和中国教育部(30770081 ) 的天津科学委员会的一般基础

英文摘要：

In order to quantitate the bovine immunodeficiency virus line （BIVL） was established by transfecting baby hamster kidney （BIV） cells infection in vitro, a BIV indicator cell with reporter plasmids containing the firefly luciferase gene driven by a BIV long terminal repeat promoter. The BIV activates promoter activity of the LTR to express luciferase upon infection. BIV infection could therefore by quantified by detection of luciferase activity. Compared to standard assays used to detect BIV infection, the BIVL-based assay is 10 times more sensitive than the the CPE-based assay, and has similar sensitivity with the viral capsid protein Western blot assay BIV indicator cell line could detect BIV infection specifically. Luciferase activity of BIV infected BIVL cells showed a time dependent manner, and 60 h post infection is the optimal time to detect BIV infection. Luciferase activity of BIVL cells correlates with the BIV capsid protein expression. Moreover, a linear relationship was found between MOI and the activated intensity of luciferase expression. In brief, the BIV indicator cell line is an easy, robust and quantitive method for monitoring BIV infection.