中文摘要：

研究被执行与砷的低集中从硫醚矿石为曲霉属菌尼日尔紧张 KBS4 检验可能性到 bioleach 金属并且优化由直角的数组优化影响这进程的参数。真菌的样品被收集，净化并且定序。bioleaching 过程与 L25 Taguchi 直角的试验性的数组设计被优化。五个因素被调查， 25 批 bioleaching 测试为每个因素在五个层次被运用。参数是为 bioleaching 的起始的 pH，粒子尺寸，肉密度，起始的接种物和住处时间。试验性的结果在优化沥滤的条件下面显示出那：pH 5.5，粒子尺寸 180 m，起始的接种物缩放 3

英文摘要：

A study was carried out to examine the possibility for Aspergillus niger strain KBS4 to bioleach metals from sulphide ore with low concentration of arsenic and to optimize the parameters that affect this process by orthogonal array optimization. Fungal sample was collected, purified and sequenced. The bioleaching process was optimized with L25 Taguchi orthogonal experimental array design. Five factors were investigated and 25 batch bioleaching tests were run at five levels for each factor. The parameters were initial pH, particle size, pulp density, initial inoculums and residence time for bioleaching. The experimental results showed that under optimized leaching conditions： pH 5.5, particle size 180 μm, initial inoculums size 3×10 7 spores per ml, pulp density 15% and residence time of 20 days, the bioleach ability of metals were 63% Fe, 68% Zn, 60% As, 79% Cu and 54% Al. The biosorption of metal ions by fungal biomass might occur during the bioleaching process but it did not hinder the removal of metal ions by bioleaching.