中文摘要：

目的：探讨维药异常黑胆质成熟剂总酚治疗人宫颈癌的可行性。方法：分别用不同浓度的异常黑胆质成熟剂总酚作用于人宫颈癌Hela细胞及SiHa细胞,于作用后24、48、72、96 h,用倒置显微镜观察细胞形态学变化,MTT比色法测定各组细胞的抑制率和半数抑制浓度（IC50）。结果：在25～125μg/mL浓度区间,总酚对Hela细胞的增殖有明显的抑制作用;在75～175μg/mL浓度区间,总酚对SiHa细胞的增殖有明显的抑制作用,且对2种细胞的抑制作用都呈明显的量效关系和时效关系（P〈0.01）;总酚作用于Hela细胞后48 h的IC50=（125.26±16.15）μg/mL,而作用于SiHa细胞后48 h的IC50=（134.51±2.55）μg/mL。两者比较差异无统计学意义（P〉0.05）;两种细胞经总酚作用后均出现明显的凋亡特征,且随着药物浓度增高及作用时间延长细胞形态学变化更明显。结论：异常黑胆质成熟剂总酚能抑制宫颈癌Hela细胞及SiHa细胞体外生长,且对两种宫颈癌细胞的抑制作用基本相同。故异常黑胆质成熟剂总酚可作为临床治疗宫颈癌的药物进一步深入研究。

英文摘要：

Objective：To explore the feasibility of total phenolics from Abnormal Savda Munziq on treating human cervical carcinoma.Methods：MTT assay was used to determine the growth inhibition ratio and IC50 on Hela cells and SiHa cells after treated with different concentrations of total phenolics.Morphologic changes of cells were observed by inverted microscope.Results： Total phenolics inhibited the proliferation of Hela cells in the concentration range of 25-125 μg /mL;Total phenolics inhibited the proliferation of SiHa cells in the concentration range of 75-175 μg/mL;The inhibitory actions of total phenolics on the Hela and SiHa appeard dose-effect relationship and time-effect relationship obviously（P〈0.01）;The IC50 of Hela was（125.26±16.15）μg/mL after 48h total phenolics treatment;The IC50 of SiHa was（134.51±2.55）μg/mL after 48h total phenolics treatment.And there was no statistical sense in the disparation of them（P〈0.05）;Both of the cells showed apoptosis character evidently after total phenolics treatment,Along with the increasing of the concentration and the action time,morphologic changes of cells were more obviously.Conclusion：Total phenolics could inhibit the growth of Hela cells and SiHa cells and the inhibitory actions of total phenolics on the two cells is almostly the same.Therefore,total phenolics from abnormal savda munziq is deserved to be further studied for treating human cervical carcinoma.