中文摘要：

目的 观察持续频闪光刺激对发育敏感期豚鼠视网膜组织及功能的影响。方法 24只出生日龄14 d的豚鼠随机分为频闪组和对照组，每组均为12只。频闪组使用频闪调光器以0.5 Hz频率等时交替频闪，照度波动0～500 Lux；对照组给予500 Lux照明。2组均使用500 nm波长发光二极管，控制光照时间为昼夜12 h轮替。实验第12周所有豚鼠行眼底彩色照相和闪光视网膜电图（F-ERG）检查。检查结束后摘除眼球，测量眼球水平径、垂直径及前后径3条径线，光学显微镜和透射电子显微镜观察眼球后极部结构改变。结果 与对照组比较，频闪组豚鼠眼底漆裂纹样改变更为明显，ERG a波潜伏期延长；眼球水平径、垂直径及前后径分别较对照组增加（0.89±0.30）、（0.69±0.20）、（0.96±0.30） mm，差异有统计学意义（t=12.7、11.9、15.8，P＜0.05）。光学显微镜观察发现，频闪组豚鼠巩膜纤维出现扩张；透射电子镜显微镜观察发现，视网膜感光细胞层外段排列稀疏紊乱并可见大量脱落外节盘膜。结论 持续频闪光刺激会诱导豚鼠眼球产生过度发育，并会影响视网膜结构与传导功能。

英文摘要：

Objective To observe the effect of persistent flickering stimulus on the structure and function of retina in guinea pigs during a developmentally sensitive period.Methods Twenty-four two- week-old guinea pigs were randomly divided into flicker light （FL） group and control group, with 12 guinea pigs in each group. Animals in FL group were raised under 500 Lux illumination with a duty diurnal cycle of 50% at a flash rate of 0.5 Hz. Animals in control group were reared under steady 500 Lux illumination. Light emitting diode （LED） lamps were used for lighting under a 12-hour light/12-hour dark cycle. After the collection of fundus photographs and electroretinograms recorded at week 12, eyeballs were taken out, three dimensions were measured, and histopathological changes were examined.Results Compared to control group, tessellated fundus in FL group appeared more prevalent; implicit time of “a” waves were prolonged in electroretinogram; the eyeballs were increased in horizontal, vertical, axial dimensions by （0.89±0.30）, （0.69±0.20） and （0.96±0.30） mm respectively, the differences between two groups were statistically significant （t=12.7,11.9,15.8;P〈0.05）. The gap of sclera collagen fiber was slightly widened.The photoreceptor layer was more likely to develop a disordered outer segment, which contained deciduous disc membranes.Conclusion Persistent flickering stimulus is attended by development of excessive ocular enlargement,which could affect the retinal structure and function of photoreceptors.