中文摘要：

为筛选更好的乙酰胆碱酯酶抑制剂，应用^1HNMR的方法研究了石杉碱甲的一个类似物——石杉碱戊与乙酰胆碱酯酶的结合性质，获得了加乙酰胆碱酯酶（[配体]：[蛋白]=1：0．005）和不加酶时石杉碱戊部分质子的非选择性、单选择性和双选择性的自旋晶格弛豫速率．加酶后质子的选择性弛豫速率变化较大，在T=298K时石杉碱戊的H—1a／H—1b质子对的分子运动相关时间τ1a、1b由不加酶时的27．7ps变化到结合酶后的11．7ns，H-2／H-3质子对的分子运动相关时间砭、3由35．2ps变化到9．46ns，由此得出石杉碱戊与乙酰胆碱酯酶有较强的结合作用．

英文摘要：

In order to search for better acetylcholinesterase （AchE） inhibitors, the binding properties of AchE with huperizine E, which is a derivative of huperzine A, were investigated by IH NMR methods. The nonselective, selective and double-selective spin-lattice relaxation rates of some protons in huperzine E were acquired in the absence and presence of AchE at a concentration ratio of [ligand] ： [protein] = 1 ： 0.005. The enhancements of selective relaxation rates of these protons were obvious after adding AchE. The molecular motional correlation times of two pairs of protons, H-1a/H-1b and H-2/H-3, in the bound state at T=298 K were 11.7 and 9.46 ns respectively, while they were 27.7 and 35.2 ps in the free state. All of these show that Huperzine E has high binding affinity with AchE.