中文摘要：

目的：构建含人GD11和GD12基因的真核表达载体进行定位和蛋白表达研究。方法：用PCR从U251细胞cDNA克隆GD11和GD12基因，构建真核表达载体pEGFP—N2-GD11和pEGFP—N2-GD12，转染HEK293T细胞。荧光显微镜观察GDII和GD12蛋白的细胞内定位，再通过SDS—PAGE和Western b1otting鉴定蛋白的表达。结果：成功克隆到1341bp和1335bp的人源GD11和GD12基因，并准确插入真核表达载体pEGFP—N2中，荧光观察这两个蛋白定位到细胞浆中，并能利用标签抗体检测到GD11和GD12的表达。结论：GD11和GD12能够定位到细胞浆，并能通过Western b1otting检测，为进一步研究GD11和GD12的功能奠定了基础。

英文摘要：

Objective： To study the localization and expression of Rab GDP dissociation inhibitor construct eukaryotic expression vector con- taining the human GDI1 and GDI2 gene. Method ：GDI1 and GDI2 gene were amplified from U251 cell cDNA by PCR, and subeloned into eukaryotic expression vector pEGFP - N2. Recombinant plasmid were transfected into HEK293T cells. Localization of GDII and GDI2 were observed through fluorescence microscopy. SDS - PAGE and Western blotting were used to identify the expression of recombinant GDI1 and GDI2. Result：Human GDI1 gene fragment of 1 341 bp and GDI2 gene fragment of 1 335bp were obtained, and was subeloned into eukaryot- ic expression vector pEGFP - N2. GDI1 and GDI2 were found localized cytoplasm, and 76kDa recombinant GDI1 and GDI2 were success- fully expression in HEK293T cells. Conelusion：GDI1 and GDI2 localized to cytoplasm and can be detected by Western blotting,laid the foundation to further study the function of GDI1 and GDI2.