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中文摘要：
摘要目的研究吡咯烷二硫代氨基甲酸（PDTC）对脂多糖（LPS）处理的雌性MRL／lpr小鼠肾脏中细胞增殖、凋亡、炎性细胞浸润以及单核细胞趋化蛋白（MCP-1）、转化生长因子B1（TGF．p1）等分子表达的影响，初步探讨PDTC拮抗LPS诱导的狼疮倾向小鼠肾脏损伤的可能作用机制。方法选择6周龄近交系MRL／lpr雌鼠28只，随机分为LPS组（按每20g体重给予0．1mlLPS溶液，给药剂量约1．0mg／kg体重）、LPS＋PDTC组[按每20g体重先给予0．1mlPDTC（60mg／kg体重），0．5h后给予0．1mlLPS溶液（1．0mg／kg体重）]、PDTC组[按每20g体重先给予0．1mlPDTC（60mg／kg体重）]和对照组（按每20g体重给予0．1ml生理盐水溶液），每组7只。采用腹腔染毒方法，每周2次，共染毒14周。所有动物在染毒14周后处死，取左侧肾脏置于10％福尔马林固定、制作石蜡切片，切4μm切片进行HE、PAS和Masson染色。在右肾切取约1mm。肾皮质置于2．5％戊二醛固定，用于制备透射电镜切片，于电镜下观察肾小球沉积物及基底膜的改变。用ELISA方法检测血清抗双链DNA（ds-DNA）、MCP-1和TGF-β1表达水平。用Westernblot的方法检测LPS和PDTC对MAPK信号通路相关蛋白pp38、p38、胞外信号调节激酶．（ERK）、磷酸化的胞外信号调节激酶（pERK）、c-Jun氨基末端激酶（JNK）、磷酸化的c-Jun氨基末端激酶（pJNK）和凋亡相关蛋白Bcl-2、Bax、Caspase3、Caspase9、核因子-κB（NF-κB）等表达的影响。用TUNEL染色检测PDTC对LPS染毒小鼠肾脏细胞凋亡的影响。用免疫组化法检测MCP-1和TGF-B1在MRL／lpr小鼠肾脏组织中的表达情况。结果动物实验结果显示，LPS染毒14周后，20周龄的雌性MRL／lpr小鼠的体重差异无统计学意义。与PDTC组小鼠相比，LPS组血清抗ds-DNA、MCP-1水平增高，TGF-β1水平下降，差异有统计学意义（P〈0．05）；与LPS组相比，PDTC干预组血清抗ds-DNA?
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