为探讨卤代酚复合污染对水生生物的联合毒性效应,探究较为可靠的检测方法,根据所选6种卤代酚化学结构的相似程度和48h LC50值,将其分为3组二元混合物进行急性暴露实验,毒性单位比为1:1,每组分2个浓度级别观察金鱼生物学行为的变化,同时检测金鱼肝脏中3种抗氧化酶(SOD,CAT和GSH—Px)活性以及酶活性影响率的变化.结果表明:3组卤代酚对金鱼肝脏中3种抗氧化酶的酶活性影响率变化均有显著性差异(P〈0.01);3种抗氧化酶中仅SOD在1/2 LC50剂量暴露组中均表现为明显升高的酶活性激活率或酶活性抑制率,提示SOD较适合作为卤代酚低浓度复合水污染情况监测的生物化学检测指标.
In order to study the joint toxicities of the analogous halophenols in aquatic biotas and to provide effective testing method, the authors divided six kinds of halophenols into three couples of equitoxic binary mixture by two exposed concentrations according to their similar structure characters and 48 h LC50. The biological behaviors' abnormity of the exposed Carassius auratus, the enzyme activity and the enzyme influence rate of the SOD, CAT and GSH-Px in their livers were observed and detected separately. The results showed that there existed significant difference among the exposed groups in the enzyme influence rate (P 〈 0.01 ) of the three antioxidant enzymes. SOD was significantly induced or inhibited in all the 1/2LC50 groups among the three enzymes, which indicated that SOD was the most suitable biochemical indicator of the low-grade water pollution of the analogous halophenols.