采用循环伏安法对从湖南省吉首大田湾污水处理厂曝气池活性污泥中富集和筛选的37株产电菌的电化学活性进行考察。结果发现,4株菌株(F012、F015、F021、F026)的电化学活性较为显著,其中F026的电化学活性最好。对4株产电菌的系统发育分析表明,菌株F012属于Dyella属,与Dyella marensis CS5-B2T系统发育关系最为密切(相似性为97.22%);菌株F015属于Paludibacterium属,与该属的Paludibacterium yongneupense 5YN8-15T系统发育关系最为密切(相似性为97.70%);菌株F021和F026都属于Pseudomonas属,分别与该属的Pseudomonas simiae OLiT和Pseudomonas otitidis MCC10330T系统发育关系最为密切(相似性分别为99.60%和98.62%)。生物学特性研究表明,电化学活性最好的产电菌F026的生长温度范围为20-40℃,最适宜生长温度为30-35℃;生长p H范围为5-9,最适p H生长范围为8-9,适合作为微生物燃料电池的高效产电菌。
Electrochemical activities of 37 electric-producing (EP) bacterial strains were enriched and screened from an aerator in Datianwan Sewage Treatment Plant located in Jishou city, Hunan Province. They were studied using cyclic volta-ampere technique. It was found in the results that four EP strains (F012, F015, F021 and F026) had fairly obvious electrochemical activities, among them strain F026 had the best. The phylogentic analyses of the four EP strains indicated that strain F012 was closely related to genus DyeUa with 16S rRNA gene sequence similarity at 97.22% (Dyella marensis CS5-B2T) ; and strain F015 belonged to the genus of Paludibacterium, and had the closest relation to Paludibacterium yongneupense 5YN8-15T of the genus with 16S rRNA gene sequence similarity at 97.70%. Strains F021 and F026 belonged to the genus of Pseudomonas, and had the closest relation to Pseudomonas simiae OLiT and Pseudomonas otitidis MCC10330y with 16S rRNA gene sequence similarity at 99.60% and 98.62% respectively. The results of biological characteristics showed that strain F026 had the best electrochemical activity and grew well at 20 - 40 ℃, with optimal temperatures at 30 - 35 ℃, pH range at 5 - 9 with optimal pH at 8 - 9, suggesting F026 is a promising efficient EP strain for microbial fuel cell (MFC).