中文摘要：

目的观察富含半胱氨酸的酸性分泌蛋白（secreted protein acidic rich in cysteine,SPARC）在具有肥胖及糖尿病表型的ob/ob鼠骨骼肌组织中的表达情况。方法选择10周龄的ob/ob小鼠（C57BL/6J-Lepob/J）与其同窝野生对照型各6只,取其后肢骨骼肌组织,采用RT-PCR方法检测骨骼肌组织中SPARC基因的表达水平,Western blot方法测定SPARC蛋白的表达水平,并用免疫荧光染色方法对骨骼肌组织进行染色观察。结果 SPARC在ob/ob鼠骨骼肌组织中呈现明显高表达。结论 SPARC可能参与了肥胖及糖尿病的发生。

英文摘要：

Objective To investigate the expression of SPARC in skeletal muscle tissues of ob/ ob mice. Methods Six ob/ob mice and 6 littermates of ten weeks old whose hindlimb muscles were taken. RT-PCR was used to detect SPARC mRNA expression in the skeletal muscle of oh/ ob mice and the littermate controls. And the differential protein expression of SPARC between the two groups was analyzed by immunofluorescence and Western blot. Results Compared with the littermates, the SPARC mRNA and protein in ob/ob mices showed higher expression. Conclusion SPARC may be involved in doesity and diabetes.