中文摘要：

目的：探讨肿瘤相关抗原线粒体蛋白质12基因（tumor-associated antigen mitochondrial protein 12 gene,TAMP12）表达变化对人宫颈癌HeLa细胞的抑制作用。方法：利用计算机辅助设计并化学合成3对针对TAMP12的siRNA片段,通过脂质体法将siRNA转染于TAMP12高表达的HeLa细胞中,筛选有效的siRNA片段。以RT-PCR、实时定量PCR及流式细胞术（FCM）检测肿瘤细胞TAMP12 mRNA及蛋白表达的变化。以激光扫描共聚焦显微镜（confocal lyser scarning microscope,CLSM）观察双色荧光标记蛋白的亚细胞定位和定量表达。以3H-TdR掺入实验和FCM检测阻断TAMP12基因表达对HeLa细胞增殖和凋亡的影响。结果：CLSM检测显示TAMP12蛋白主要表达在HeLa细胞线粒体中。转染siRNA-TAMP12的肿瘤细胞中TAMP12 mRNA及蛋白的表达显著降低,与对照组对比,抑制率分别为81%和87%（P〈0.01）;转染细胞的DNA合成受抑制,抑制率达42%（P〈0.01）;转染siRNA-TAMP12肿瘤细胞的凋亡率由对照细胞的8.14%上升到15.59%（P〈0.01）。结论：RNA干扰可阻断TAMP12基因的表达,从而对人宫颈癌Hela细胞产生抑制作用。

英文摘要：

Objective： To explore the inhibitory effects of tumor associated antigen mitochondrial protein 12 gene （TAMP12） on human cervical cancer cell line HeLa. Methods： Three pairs of TAMP12-specific small interference RNA （siRNA） fragments were designed and synthesized. The siRNA fragments were transfected into HeLa cells highly expressing TAMP12 gene via liposome and the effective siRNA fragment was selected. The expression of TAMP12 mRNA and protein was detected by RT-PCR and flow cytometry （FCM）. The subcellular localization and quantification of TAMP12 protein labeled with double fluorescein were observed under laser scanning confocal microscope （CLSM）. 3H-TdR incorporation and FCM were used to analyze the proliferation and apoptosis of HeLa cells after interference of the TAMP12 gene expression. Results： CLSM observation showed that TAMP12 protein was mainly expressed in mitochondria of HeLa cells. Cells transfected with siRNA-TAMP12 significantly suppressed the expression of TAMP12 mRNA and protein（ the inhibitory rates were 81% and 11.23%, respectively）and DNA synthesis in vitro（the inhibitory rate was 42% ）. Moreover, transfection with siRNA-TAMP12 increased the apoptosis rate from 8.14% in the control group to 15.59%. Conclusion： Blocking the TAMP12 gene expression may inhibit the growth of Hela cells.