中文摘要：

以毛尖紫萼藓（Grimmia pilifera P.Beauv.）配子体为试材,以Benek、MS、Knop 3种培养基为基本培养基,通过预培养和预处理,借助旋流振荡器,对毛尖紫萼藓组织培养消毒方法及接种培养基类型进行了筛选。结果表明：预培养15d左右的外植体,0.025%升汞溶液旋流振荡105s能有效地对外植体进行消毒,Benek培养基适合毛尖紫萼藓返绿生长。

英文摘要：

Chosen Grimmia pilifera gametophytic as test material,Benek,MS,Knop as basic culture medium,the tissue culture of disinfection method and inoculation medium types were screened,through pre-culture,pretreatment,had the aid of swirl oscillator.The results indicated that explants pre-culture for about fifteen days,swirl oscillating by 0.025% mercuric chloride solution for 105 seconds cound disinfect effectively for explants.Benek culture medium was suitable for Grimmia pilifera to return to green growth.