分析不同时间的50%甲醇老化处理对玉米种子发芽和幼苗生长的影响,明确甲醇老化处理的最佳时间。结果表明,与蒸馏水浸泡40 min(CK)相比,50%甲醇老化处理10-30 min时种子发芽指标(活力指数除外)均无显著变化;40 min时种子发芽率、发芽势、发芽指数、活力指数分别降低5.67%、8.67%、11.08%和41.22%;50 min时分别降低6.00%、10.00%、18.05%和47.70%;60 min时分别降低21.33%、28.00%、35.62%和61.80%;70 min时种子发芽指标降至较低水平。甲醇老化处理10 min时,玉米幼苗苗高、根长、根苗比、苗重和根重均显著降低,且降低幅度高于发芽指标。与CK相比,甲醇老化处理10-30 min时对种子脱氢酶活性和相对电导率无显著影响,40-70 min时脱氢酶活性显著降低,相对电导率显著增加。因此,50%甲醇老化处理应选择40-60 min为宜。
The effects of aging treatment with 50% methanol of different time on maize seed germination and seedling growth were analyzed to know the appropriate aging time, The results indicated that compared with CK, ag- ing treatment of 10-30 minutes didn't affect seed germination indexes(except for vigor index) significantly. Seed germination percentage, germination energy, germination index and vigor index after aging treatment of 40 minutes were decreased by 5.67%, 8.67%, 11.08% and 41.22%, respectively; these indexes after aging treatment of 50 min- utes were decreased by 6.00%, 10.00%, 18.05% and 47.70%, respectively; these indexes after aging treatment of 60 minutes were decreased by 21.33%, 28.00%, 35.62% and 61.80%, respectively. Seed germination indexes were very low after aging treatment of 70 minutes. Aging treatment of 10 minutes decreased shoot height, root length, root- to-shoot ratio, shoot weight and root weight. Aging treatment with methanol inhibited seedling growth much more than seed germination. Compared with CK, aging treatment of 10-30 minutes didn't affect dehydrogenase activity and relative electrical conductivity, and aging treatment of 40-70 minutes decreased dehydrogenase activity and in- creased relative electrical conductivity significantly. Therefore, 40-60 minutes is the appropriate aging time with 50% methanol.