中文摘要：

目的：探明生防菌株巨大芽孢杆菌RBIO的发酵培养条件，提高其抑菌活性。方法：首先采用麸皮和豆粕作为供试菌的碳氮源，通过单因素（C源、N源）优化实验得出豆粕1．0％、麸皮1．5％的条件下具有较好抑菌效果。采用Plaekett-Burman设计筛选出主要影响因素为麸皮、豆粕和pH。最后再进行Box-Benhnken（响应面）试验分析。结果：RB10的最佳发酵培养基配方：麸皮0．98％、豆粕1．14％、初始pH7．39；最佳发酵条件：接种量1．5％、温度39℃、发酵时间78h、转速190r／min。在此发酵条件下，拮抗细菌RB10的抑菌率为69．416％，比之前采用正交试验优化发酵培养条件后的抑菌活性提高了28．524％，与理论值69．974％相差0．797％（理论误差〈1％），实验值与预测值基本相符。结论：响应面法优化出的RB10的发酵培养条件参数准确可靠，证实了模型的有效性。

英文摘要：

Objective：Response surface method （RSM） was used in order to optimize the fermentation conditions of Megaterium Bacillus RB10 and improve the inhibition rates. Method： The C source concentration （bran 1.5% ） and N source concentration （bean cake 1.0% ） were determined through single -factor experiments. The major factors including bran, bean cake and initiation pH were screened with Plackett - Burman expermiental design. Result ：The best formulation （ bran 0. 98% , bean cake 1.14% , initiation pH 7.39 ） of fer- mentation medium and the best fermentation condition （inoculum 5%, culture temperature 39℃, culture time 78h, rotary speed 190r/ min） were confirmed after above major foctors optimized by Box - Behnken expermiental analysis. Under the best fermentation condition, the theoretical value of inhibition rate was 69. 974%. And the expermiental value of inhibition rate was 69. 416%, which was 28. 524% higher than initial medium. The difference between expermiental value and theoretical value was 0. 797% （relative error 〈 1% ）, which suggested that expermiental value matched well with the equation. Conclusion：The optimum conditions of RB10 from ILSM were validity and confirmed the statistical significance of the model.