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Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones
  • ISSN号:0253-505X
  • 期刊名称:Acta Oceanologica Sinica
  • 时间:0
  • 页码:62-69
  • 分类:Q959.7[生物学—动物学] TQ014[化学工程]
  • 作者机构:[1]State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China, [2]College of Marine Science, Shandong University at Weihai, Weihai 264209, China
  • 相关基金:Foundation item: The State Key Laboratory of Microbial Technology under contract No. M2010-04; Independent Innovation Foundation of Shandong University (IIFSDU); the National Natural Science Foundation of China under contract No. 40730847; Special Non-profit Research Project from Ministry of Agriculture of the Peoples Republic of China under contract No. nyhyzx 07-046.
  • 相关项目:渤海中南部底栖生物生产过程与生物多样性集成研究
中文摘要:

研究被承担调查与海海葵联系的不是自养的细菌的植物群。海海葵 Anthopleura midori 的样品从 Weihai 的海岸被收集,细菌从这些样品被孤立。另外,可行细菌的高数字从 celom 墙和海葵的表面被获得,可耕种的细菌的社区是很多样的。由于这隔离, 60 紧张被获得,他们中的 56 个被测试的 16S rRNA 基因顺序分析和有限 phenotypic 为鉴定和描述选择。在这些之中孤立, 16 紧张是种系发生地与类 Pseudoalteromonas 和附近的 taxa 的成员有关。其它孤立类 Colwellia Vibrio Acinetobacter Pseudomonas Endozoicomonas, Roseovarius, Paracoccus, Loktanella, Leisingera, Sulfitobacter,杆菌,葡萄球菌, Plantibacter, Microbacterium,小球菌, Joostella, Psychroserpens, Cellulophaga, Krokinobacter, Polaribacter 和 Psychrobacter 的包括的成员七潜在的新奇的种被发现。在 60 紧张之中,他们中的 17 个能生产解朊的外酵, 20 能生产分解脂肪的外酵。紧张 NQ8 在一些 Vibrio 紧张上有强壮的对抗效果。这研究证明从海海葵 Anthopleura midori 的细菌的 culturable 部分是多样的并且看起来为新奇 bioactive 材料的发现作为来源拥有许多潜力。

英文摘要:

A study was undertaken to investigate the heterotrophic bacterial flora associated with the sea anemones. Samples of the sea anemones Anthopleura midori were collected from the coast of Weihai and bacteria were isolated from these samples. Additionally, high numbers of viable bacteria were obtained from the celom wall and surface of anemone, the community of cultivable bacteria was very diverse. As a result of this isolation, 60 strains were obtained, 56 of them were selected for identification and characterization by 16S rRNA gene sequence analysis and limited phenotypic testing. Among these isolates, 16 strains were phylogenetically related to members of the genus Pseudoalteromonas and neighboring taxa. Other isolates included members of the genera Colwcllia, Vibrio, Acinetobactcr, Pseudomonas, Endozoicomonas, Roseovarius, Paraeoceus, Loktanella, Leisingcra, Sulfitobacter, Bacillus, Staphylococcus, Plantibacter, Microbaeterium, Micro- coccus, Joostella, Psychroserpens, Cellulophaga, Krokinobaeter, Polaribacter and Psychrobaeter. Seven potential novel species were found. Among 60 strains, 17 of them can produce proteolytic exoenzyme, 20 can produce lipolytic exoenzyme. Strain NQ8 has strong antagonistic effects on some Vibrio strains. This study demonstrates that the culturable fraction of bacteria from the sea anemones Anthopleura midori is diverse and appears to possess much potential as a source for the discovery of novel bioactive materials.

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期刊信息
  • 《海洋学报:英文版》
  • 主管单位:
  • 主办单位:中国海洋学会
  • 主编:潘德炉
  • 地址:北京海淀大慧寺路8号
  • 邮编:100081
  • 邮箱:hyxbe@263.net
  • 电话:010-62179976
  • 国际标准刊号:ISSN:0253-505X
  • 国内统一刊号:ISSN:11-2056/P
  • 邮发代号:82-285
  • 获奖情况:
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),荷兰文摘与引文数据库,美国剑桥科学文摘,美国科学引文索引(扩展库),英国科学文摘数据库,英国动物学记录
  • 被引量:331