中文摘要：

【目的】研究热应激对机体（肝脏）以及生殖系统氧化、卵母细胞和附植前胚胎氧化及发育能力的影响,探讨热应激影响卵母细胞及附植前胚胎发育能力的机制。【方法】分别用总谷胱甘肽和微量丙二醛测定试剂盒检测热应激后小鼠卵母细胞、附植前胚胎和组织器官内总谷胱甘肽（totalGlutathione,TGSH）、丙二醛（MDA）的含量,并且观察后续胚胎的发育情况。【结果】母体经35℃处理6和12h后,其体内的卵母细胞（6h）和发育至1～3d的胚胎（桑椹胚前期,12h）的后续胚胎发育率显著降低,胚胎中TGSH含量下降（P〈0.01）、MDA含量升高（P〈0.01）,卵母细胞中二者的含量未有变化;发育至第4天、第5天的胚胎（桑椹胚和囊胚）中TGSH和MDA的含量及胚胎发育率均未有变化（P〉0.05）;发育至第1～5天的体外培养的胚胎分别经39℃处理12h后,胚胎中TGSH和MDA及其发育率均未有变化（P〉0.05）;随着热应激温度升高和时间的延长,肝脏和输卵管中TGSH显著降低、MDA显著升高;当温度达到41℃时,卵巢和子宫中TGSH显著降低、MDA显著升高。【结论】桑椹胚以前的胚胎遭受母体热应激后,发育能力降低,这与热应激所诱导的氧化损伤密切相关,但是母体热应激后卵母细胞后续胚胎的发育能力降低与氧化损伤无关。体外培养的胚胎经39℃处理12h后,对附植前胚胎氧化损伤和孵出率均无影响。

英文摘要：

[Objective] This study was conducted to determine the effects of heat stress on the oxidate damage of organism（liver）, genital system, oocytes and preimplantation embryos, and to observe oocytes＇ and embryos＇ subsequently developmental competence. [ Method ] TGSH and MDA in the oocytes, preimplatation embryos and several organs were determined by using total glutathione and malonaldehyde assay kit, respectively. The subsequently developmental competence of heat-stressed oocytes and embryos was determined. [Result] The subsequently developmental competence of the oocytes （6 h） and embryos （12 h） on day 1 to day 3 （before morula） in the mice heat-stressed at 35℃ for 6 h or 12 h decreased significantly. The TGSH content decreased and MDA output increased significantly in heat-stressed embryos on days 1, 2 and 3, but not in heat-stressed ooeytes. However, nothing changed in embryos on days 4 and 5 of age cultured in vivo and the embryos on days 1 to 5 of age heat-stressed at 39℃ for 12 h cultured in vitro. The 7GSH content increased and MDA output decreased significantly in liver and oviduct with the increased grading of heat stress and prolonged time, and in the ovary and uterus with 41℃ heat stress only. [ Conclusion ] The results demonstrate that the loss of developmental competence of early embryos is associated with oxidate damage of maternaUy heat-stressed enbryos before morula, but neither of maternally heat-stressed oocytes nor in vitro cultured 1 to 5d embryos.