中文摘要：

目的：体外研究罗非鱼鱼皮胶原多肽对人结肠癌细胞系LoVo增殖能力的影响,并初步探讨其作用机理。方法：体外培养人结肠癌LoVo细胞,噻唑蓝（methylthiazolyldipHenyl-tertrazolium bromide,MTT）法检测罗非鱼鱼皮胶原多肽对细胞增殖的影响;荧光探针染色法观察细胞膜及核的变化;激光共聚焦显微镜观察细胞内活性氧（reactive oxygen species,ROS）表达;比色法检测细胞线粒体呼吸链酶复合物Ⅰ和Ⅲ的活性。结果：罗非鱼鱼皮胶原多肽对LoVo细胞的增殖具有一定的抑制作用,当质量浓度为100 mg/m L、作用48 h时,抑制率达到55.03%（P〈0.05）;与空白对照组相比,经胶原多肽作用后,LoVo细胞核浓缩,细胞膜出现不完整,细胞凋亡;且细胞内活性氧水平表达显著增加（P〈0.05）,细胞内线粒体呼吸链复合物Ⅲ的活性显著降低（P〈0.05）,但对线粒体呼吸链复合物Ⅰ的活性无影响。结论：罗非鱼鱼皮胶原多肽对人结肠癌细胞具有一定的增殖抑制作用,可能与抑制线粒体呼吸链复合物Ⅲ活性,使胞内ROS水平增加,破坏细胞膜形态有关。

英文摘要：

Objective： To investigate the effects of collagen peptides from tilapia skin on the proliferation ability of human colon carcinoma cell line LoVo in vitro and its possible mechanism. Methods： The inhibitory effects of collagen peptides from tilapia on the proliferation of LoVo cells were observed by MTT assay. Fluorescent staining method was used to analyze the effect on cell membrane and nucleus. Laser confocal microscopy was used to examine reactive oxygen species（ROS） expression in LoVo cells. The activities of mitochondrial respiration chain complex I and III were analyzed by colorimetry. Results： Collagen peptides from tilapia skin showed certain inhibitory effect on the proliferation of LoVo cells. After being treated by 100 mg/m L of collagen peptides for 48 h, the growth inhibitory effect rate reached 55.03%（P〈0.05）. Compared with the control group, the nucleus of LoVo cells was shrunk and cell membrane was destructed after being treated by collagen peptides. Meanwhile, the ROS level was significantly increased（P〈0.05）. Collagen peptides significantly inhibited the activity of complex III. Conclusion： Collagen peptides from tilapia skin have inhibitory effects on the proliferation of human colon carcinoma cells, which is probably associated with the inhibited activity of mitochondrial respiration chain complex III in LoVo cells, promoting ROS expression and destructing the cell membrane in LoVo cells.