欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的：筛选出分离纯化槲寄生总黄酮的最佳树脂,并对影响分离纯化的因素进行研究,得到优化的纯化条件。方法：选择了四种大孔吸附树脂（AB-8、NKA-9、NKA-Ⅱ和D101）用来分离纯化槲寄生中的总黄酮,采用动态吸附-解吸方法,利用分光光度法测定总黄酮的含量,研究不同的大孔吸附树脂及其不同的工艺条件对总黄酮分离纯化的影响。结果：AB-8分离效果最好,其最佳工艺为上柱原液pH值4左右,上柱速度2BV/h,以40%乙醇为洗脱液控制洗脱液流速1BV/h,洗脱液用量为4BV。经AB-8纯化后,槲寄生产品中黄酮的纯度由12.16%提高到43.56%。结论：AB-8大孔树脂可以较好地分离纯化槲寄生黄酮。
英文摘要：
Objective： To choose the optimum resin for separation and purification of mistletoe （Viscum album） flavonoids and to analyze influenceable factors of separation and purification process. Methods： Four macroporous resins including AB-8, NKA-9, NKA-Ⅱ and D101 were used to separation of total flavonoids of mistletoe （Viscum album）. During the adsorption and desorption process, with UV to assay total flavonoids, the different macroporous resins and their capacities were studied. Results： AB-8 resin possesses much higher adsorption and desorption capacity. The optimum conditions are： the feed solution pH 4, the flow rate 2 BV/h, 40% alcohol as eluant for desorption, the flow rate 1 BV/h and eluant volume 4 BV. An enriched total flavonoids extract with 43.56% purity can be separated from crude mistletoe extract （about 12.16% purity） in one run. Conclusion： It is feasible to separate and purify mistletoe flavonoids with AB-8 resin.
同期刊论文项目
同项目期刊论文
期刊信息
位置：