中文摘要：

以红肉苹果‘紫红3号’（新疆红肉苹果与‘富士’F1代）的红色幼嫩叶片为外植体诱导的红色愈伤组织为试材,初步探讨生长素调控苹果花青苷代谢机理。根据拟南芥AtARF3蛋白序列在苹果基因组中Blast比对得到一个生长素信号相关基因（MDP0000173151）,暂命名为MdARF3。克隆测序发现该基因的开放阅读框长度为2 127 bp,编码708个氨基酸。进化树分析表明,MdARF3与AtARF3在同一进化支上,推测它们具有相似的功能。愈伤组织在含有0.3 mg·L-1 NAA的MS培养基培养2 h之后其MdARF3上调表达,表达量极显著高于无NAA培养基（对照）,而CHS、CHI、F3H、DFR、UFGT及LDOX等花青苷合成结构基因的表达量均极显著低于对照,并且MdARF3表达量与培养基中NAA浓度呈显著正相关（相关系数为0.98）,与愈伤组织花青苷含量呈显著负相关（相关系数为-0.89）,推测MdARF3对培养基中生长素快速做出反应调控花青苷合成;通过原核诱导获得了MdARF3的重组蛋白,为进一步研究MdARF3蛋白在花青苷代谢途径中的功能奠定了基础。

英文摘要：

The red-callus induced from the leaves of‘Zihong 3’apple in an F1 population of Malus sieversii f. niedzwetzkyana crossed with‘Fuji’was used as materials. An auxin signal relative gene（MDP0000173151）was obtained from apple genome by BLAST searches of the Arabidopsis AtARF3 protein sequence,designate MdARF3. Sequence analysis indicated that the open reading frame（ORF）length of MdARF3 was 2 127 bp,which encoded 708 amino acids. Phylogenetic analysis revealed that MdARF3 was homologous with AtARF3 which was involved in the auxin signaling. Compared with the NAA-deprived callus（the control）,the transcript level of MdARF3 was significantly higher on the MS medium contained 0.3 mg · L-1 NAA after 2 hours. In contrast,the expression levels of anthocyanin biosynthesis structural genes（CHS,CHI,F3H,DFR,UFGT and LDOX）were lower on the MS medium contained 0.3 mg · L-1 NAA. At the 0.05 level,the expression of MdARF3 was positively correlated with the auxin concentration（correlation coefficient 0.98）and was negatively correlated with the anthocyanin content（correlation coefficient–0.89） of callus. Therefore,the MdARF3 is supposed to be an auxin-responsive transcription factor to regulate the biosynthesis of anthocyanin in apple. The recombinant protein of MdARF3 was obtained by the prokaryotic expression technique,which laid a foundation for functional identification of MdARF3 associated with anthocyanin metabolism.