利用硼掺杂金刚石(BDD)电极通过循环伏安法和微分脉冲伏安法研究了阿昔洛韦在0.10mol/L磷酸盐缓冲溶液(pH7.4)中的电化学行为及其与DNA的相互作用。与玻碳电极相比,阿昔洛韦在BDD电极上的循环伏安曲线任1.17V处的氧化峰电流更人,背景电流较低。根据峰电位随溶液pH值和扫描速率的变化趋势考察了阿昔洛韦的电化学氧化机理,认为阿昔洛韦在BDD电极上的电化学氧化过程有2个电子和2个质子参与。对不同比例阿昔洛韦和DNA相互作用的研究表明,两者的相互作用模式为插入模式,结合位点数为1.07,结合常数为4.47×10^6,并通过阿昔洛韦与同浓度单、双链DNA的相互作用得到验证。
The electrochemical behavior of acyclovir on the boron-doped diamond( BDD) electrode and its interaction with DNA in 0. 10 mol/L phosphate buffer solution( pH 7.4) were studied by cyclic voltammetry and differential pulse vohammetric method. Compared with that on glassy carbon electrode, an oxidation peak of acyclovir at 1.17 V with lower background current and higher peak current was observed on the BDD electrode. The electrochemical oxidation mechanism of acyclovir on the BDD electrode was investigated by the relationships of tile oxidation peak potential with the scan rate and pH value, and 2-electron-transfer and 2-proton-participation in the oxidation process of acyclovir were found. The study of interaction between different proportions of acyclovir and DNA indicated that acyclovir interacted DNA based on inset mode with binding site of 1.07 and binding constant of 4. 47 ×10^6. The above results were further verified by the study of the interactions of acyclovir with single-stranded DNA and double-stranded DNA, respectively.