中文摘要：

利用PCR技术扩增小鼠高迁移率族蛋白1（HMGB1）基因启动子序列，构建小鼠HMGB1启动子荧光素酶报告基因pGL3-basic-HMGB1．经PCR、酶切及测序鉴定后，用脂质体法将pGL3-basic-HMGBl转入巨噬细胞264．7中，并应用萤光素酶测定系统检测其活性．检测结果显示pGL3-basic—HMGB1具有启动子活性．小鼠HMGB1启动子荧光素酶报告基因pGL3-basic—HMGB1的成功构建，为进一步研究HMGB1提供基本材料．

英文摘要：

In order to construct the luciferase reporter gene containing mouse HMGB1 promoter pGL3-basicHMGB1, the promoter of high mobility group box I（HMGB1 ） gene was amplified from mouse genomic DNA by Polymerase Chain Reaction （PCR）. The recombinant PGL3-basic-HMGB1 was transfected into 264.7 ceils by lipofectamine after it was confirmed by PCR analysis, restriction enzyme digestion, and DNA sequencing, then the activity of luciferase was detected. The result demonstrated that the PGL3-basic- HMGB1 had the promoter activity. The luciferase reporter gene containing mouse HMGB1 promoter pGL3- basic-HMGB1 is constructed successfully, and it will become essential material for further studv.