中文摘要：

目的探讨滋养细胞分泌14—3—3 ι蛋白对子宫内膜基质细胞容受性分子整合素αvβ3表达的影响。方法RNA干扰技术下词人绒癌滋养细胞株Bewo细胞14—3—3ι蛋白的表达。Westernblot法检测Bewo细胞及培养液中14—3—3 ι蛋白的表达。建立14—3—3 ι蛋白表达下调的BeWo细胞与人子宫内膜基质细胞（endometrialstromalcells，ESC）的共培养体系，Westernblot法检测共培养体系培养液中14—3—3 ι蛋白的表达，以及ESC容受性分子整合素αvβ3的表达变化。结果与siRNA阴性对照组相比，特异性siRNA显著下调BeWo细胞和培养液中14-3—3 ι蛋白的表达（P〈0．05）。共培养体系中，与siRNA阴性对照组相比，与14—3—3 ι蛋白表达下调的Bewo细胞共培养的ESC整合素αvβ3的表达显著上调（P〈0．05），提示ESC容受性增加。与单独培养的ESC相比，培养液中添加143—3 ι重组蛋白的ESC培养24h后整合素αvβ3的表达显著下调（P〈0．05），提示ESC容受性降低。结论14-3—3 ι蛋白可以被滋养细胞分泌至细胞外，并可能发挥调控ESC容受性的作用，但其作用机制仍需进一步研究。

英文摘要：

Objective To investigate the effects of 14-3-3 ι protein secreted by human trophoblast cells on the expression of integrin αvβ3 protein in human endometrial stromal cells. Methods Small interference RNA （siRNA） targeting 14-3-3 ι mRNA was transfected into human trophoblast cell line （BeWo cells）. The expression of 14-3-3 ι protein in the BeWo cells and its culture-medium were detected by Western blot respectively. Then the BeWo cells transfected with 14-3-3 ι siRNA were co-cultured with human endometrial stromal cells （ESC）. The expression of 14-3-3 ι protein in the co-culture-medium and integrin αvβ3 protein in ESC were detected by Western blot respectively. Results Compared with negative control of siRNA, the expression of 14-3-3 ι protein in both BeWo cells and culture-medium were significantly inhibited by 14-3-3 ι siRNA. In the co-culture system, BeWo cells down-regulated by 14-3-3 ι siRNA resulted in an increased expression of integrin αvβ3 protein in ESC （P〈0.05）. The expression of integrin αvβ3 protein was significantly inhibited in the ESC co-cultured with human 14-3-3 ι recombinant protein （P〈0.05）. Conclusions 14-3-3 ι protein can be secreted by human trophoblast cells, and may regulate the receptivity of human endometrial stromal cells.