中文摘要：

目的：建立Aurora-A高表达的稳定细胞系，并探讨Aurora-A高表达对食管癌细胞侵袭和MMP-9蛋白表达与活性的影响。方法：利用脂质体法稳定转染Aurora-A表达质粒，通过Western blot和RT-PCR鉴定Aurora-A高表达的稳定细胞系。利用细胞体外侵袭实验观察细胞的侵袭能力，并通过Western blot、ELISA和明胶酶谱的方法观察MMP-9的蛋白表达水平和活性。结果：建立了Aurora-A高表达的稳定细胞系，且Aurora-A高表达能增加食管癌细胞的体外侵袭能力，并提高了MMP-9的蛋白表达水平和活性。结论：初步阐明了Aurora-A促进食管癌细胞侵袭的作用以及分子机制，为Aurora-A可能成为治疗食管癌的有效靶点提供了依据。

英文摘要：

Objective：To establish Aurora-A overexpressing transfectant clones and investigate its effect on invasiveness as well as protein expression and activity of MMP-9 in esophageal carcinoma cells. Methods：The Aurora-A expressing plasmid was transfected using Lipofectamine？ Llpofectamme 2000 and the identification of transfectedclones was confirmed by Western blot analysis and RT-PCR. The invasive properties of cells were examined by an in vitro Boyden chamber invasion assay. Level of MMP-9 protein expression and activity were measured with Western blot and ELISA as well as gelatin zymography analysis. Results：Aurora-A overexpressing transfectant clone was established, which increased cell invasion as well as protein expression and activity of MMP-9. Conclusion：The elucidation the molecular mechanism for the promotion of invasion in esophageal carcinoma by Aurora-A may lead to the development of new target for effective therapy.