中文摘要：

检测了水体铜（Cu^2＋）暴露对中华倒刺鲃（Spinibarbus sinensis）幼鱼的急性毒性以及组织铜富集的影响。结果发现：1）中华倒刺鲃幼鱼死亡率随水体铜含量和暴露时间的增加而上升（p〈0.05）,死亡率与暴露时间的关系呈＂S＂型逻辑回归曲线;铜暴露96、120、144、168h的半致死含量LC50分别为0.500、0.401、0.356和0.352mg·L^-1,铜对中华倒刺鲃幼鱼的安全含量为0.050mg·L^-1;0.4、0.6、0.8、1.0、1.2、1.4mg·L^-1铜暴露下幼鱼的半致死时间LT50分别为116.0、78.0、80.7、56.0、46.7、46.0h。2）中华倒刺鲃幼鱼组织按铜富集含量由高到低顺序排列为肝脏、鳃、肌肉,急性铜暴露（7d）不足以产生较强的富集效应。研究提示水体中的铜含量变化显著作用于中华倒刺鲃幼鱼的存活率,但对组织铜富集的影响不显著。

英文摘要：

The effects of Cu^2＋ on acute toxicity and tissue copper accumulation in juvenile qingbo,Spinibarbus sinensis,were measured.The results showed that the fish lethal rate rose with the increased of copper concentration and exposure time（p0.05）.The relationship between lethal rate and exposure time showed ＂S＂-type logic regression curve.After exposed over 96,120,144 and 168 h,the copper concentration lethal for 50%（LC50） of the fish were 0.500,0.401,0.356 and 0.352 mg·L^-1,respectively.The safe concentration of Cu^2＋ was 0.050 mg·L^-1.Exposed the fish to 0.4、0.6、0.8、1.0、1.2、1.4 mg·L^-1,the exposure time lethal for 50%（LT50） of the fish were 116.0,78.0,80.7,56.0,46.7 and 46.0 h,respectively.Accumulation of copper in tissues was highest in liver,followed by gill,and the lowest in muscle.The values of the bio-enrichment coefficient suggested that acute copper exposure was not sufficient to generate strong enrichment effect on fish.Taken together,all of results indicated that copper concentration in water could impact on the survival rate significantly while not on the copper accumulation in tissues of juvenile qingbo,Spinibarbus sinensis.