中文摘要：

目的：采用液相色谱-串联质谱（LC-MS/MS）建立人血浆中氯吡格雷活性代谢产物浓度测定的方法。方法：血浆经冷冻干燥处理,以Accucore C18为色谱柱;乙腈（含0.01%甲酸）-水（含0.01%甲酸）为流动相,流速0.4 mL·min^-1,柱温30℃,进样量2μL;通过正离子模式多反应监测扫描分析,离子通道分别为氯吡格雷活性代谢产物衍生物（CAMD）m/z 504.2→155.1,内标氯雷他定m/z382.8→336.9。结果：血浆中氯吡格雷活性代谢产物衍生物在0.5-500 ng·mL^-1范围内线性关系良好;最低检测限为0.5 ng·mL^-1;提取回收率为83.81%-97.65%;日内和日间精密度的RSD均小于9.86%。结论：本方法准确可靠,简便快速且灵敏度高,适用于人血浆中氯吡格雷活性代谢物的测定,可进一步用于研究氯吡格雷活性代谢产物的药代动力学特征。

英文摘要：

OBJECTIVE To develop a LC-MS/MS method for the determination of clopidogrel active metabolites in human plasma.METHODS The plasma was dried. The analytical column was packed with accucore C18. The mobile phase was acetonitrile （0.01% formic acid） and water （0.01% formic acid） at a flow rate of 0.4 mL·min^-1, the column temperature was 30℃ and the injection volume was 2 μL with electrospray positive ionization （ESI＋） and multiple reaction monitoring （m/z 504.2→155.1 （CAMD） and m/z 382.8→336.9 （loratadine）. The concentration of CAMD in plasma was detected by this LC-MS/MS method.RESULTS Excellent liner relationship was obtained from the range of 0.5-500 ng·mL^-1, the limit determination of CAMD was 0.5 ng·mL^-1. The average extraction recovery was 83.81%-97.65%. The inter-and intra-day RSD was less than 9.86%.CONCLUSION The method is accurate, simple and rapid with high sensitivity, which can be used to analyze clinical samples and determine the pharmacokinetics of clopidogrel active metabolites.