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中文摘要：
目的：用纳升电喷雾串联质谱（nanoESI—MS／MS）鉴定吗啡依赖相关蛋白。方法：采用吗啡递增给药法建立吗啡依赖模型，用纳洛酮催促后出现典型的戒断症状，以盐水组为对照，对长期接受吗啡处理和纳洛酮催促戒断这2种状态的大鼠纹状体进行了比较蛋白质组研究，确定了33个差异点。其中差异点150和209用电喷雾串联质谱进行了氨基酸序列分析。结果：电喷雾串联质谱分析表明差异点150和209为2个重要的吗啡依赖蛋白质：G蛋白β1亚基和电压依赖型阴离子通道蛋白1（VDAC-1），并且这2个蛋白质都发生了N端乙酰化修饰。结论：纳升电喷雾串联质谱分析是研究蛋白质N端乙酰化的好方法。N端乙酰化对蛋白质生物学功能有重要作用。这2个蛋白质N端乙酰化的生物学功能及其与吗啡依赖的关系值得深入研究。
英文摘要：
Objective：To characterize morphine dependence differential proteins by nano electrospray tandem mass spectrometry （ nanoESI - MS/MS）. Method： The rats were treated with ascending doses of morphine to achieve dependence and leading to characteristic withdrawal syndromes after naloxone - precipitated withdrawal. Comparative proteomic analysis was performed in striatum of two phases of morphine dependence, long - term exposure to morphine and drug withdrawal and 33 different protein spots were identified by matrix - assisted laser desorption/ionization - time - of - flight - mass spectrometry （ MALDI - TOF - MS）. The differential spot 150 and 209 were analyzed by nanoESI - MS/MS since they were not identified by MALDI - TOF - MS unambiguously. Results： NanoESI - MS/MS analysis indicated that spot 150 and 209 were G proteinβ1 subunit and voltage- dependent anion -selective channel protein 1 （ VDAC - 1 ） respectively. Most importantly, nanoESI - MS/MS analysis showed that the two proteins are all N - terminally acetylated. Conclusion： NanoESI - MS/MS is a powerful tool for N - terminal acetylation analysis. N - terminal acetylation is critical to biological function of proteins. It is worth of giving insight into function of the two N - terminal acetylated proteins and their relations to morphine dependence.
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