中文摘要：

背景：课题组采用新的重组腺病毒介导的基因表达手段,与传统方法比较具有高效、方便、安全等优点,可作为进入临床基因治疗应用的潜在有力手段。目的：比较腺病毒介导的人骨形态发生蛋白9（Adv-h BMP-9）与腺病毒介导的人骨形态发生蛋白2（Adv-h BMP-2）分别复合纳米羟基磷灰石/聚酰胺多孔支架材料修复重建桡骨缺损的效果。方法：新西兰白兔36只,随机分成3组,制成双侧桡骨中段13mm骨缺损模型。分别植入Adv-h BMP-9＋纳米羟基磷灰石/聚酰胺骨、Adv-h BMP-2＋纳米羟基磷灰石/聚酰胺骨、Adv-GFP＋羟基磷灰石/聚酰胺骨。植入后进行大体观察、X射线摄片、组织学检测。结果与结论：BMP-9组骨缺损完全修复,BMP-2组骨缺损部分修复,而GFP对照组骨缺损修复明显欠佳。提示重组腺病毒介导的BMP-9对桡骨骨缺损后的成骨修复作用强于BMP-2。

英文摘要：

BACKGROUND：Compared with the traditional method,this paper applies a new method of recombinant adenovirus-mediated gene expression with the advantages of high efficiency,convenience and safety,which can serve as powerful means for gene therapy.OBJECTIVE：To compare the effect of adenovirus-mediated human bone morphogenetic protein 9（Adv-hBMP-9） and adenovirus-mediated human bone morphogenetic protein 2（Adv-hBMP-2） nano-hydroxyapatite /polyamide complex on reconstruction of bone defects.METHODS：A total of 36 New Zealand rabbits were randomly divided into 3 groups and prepared for 13-mm bone defect models at the bilateral middle radius.Adv-hBMP9 ＋ nano-hydroxyapatite /polyamide bone,Adv-hBMP-2 ＋ nano-hydroxyapatite /polyamide bone,and Adv-GFP ＋ hydroxyapatite /polyamide bone were implanted into the defects in each group.General observation,X-ray photographs and histological sections were carried out separately.RESULTS AND CONCLUSION：Bone defects were completely repaired in the BMP-9 group;partly repaired in the BMP-2 group;however,reparation of bone defects was obviously poor in the GFP control group.The results revealed that effect of recombinant Adv-hBMP-9 is stronger than Adv-hBMP-2 on repairing osteogenesis after radial defects.