中文摘要：

目的：研究从昆虫美洲大蠊和斑蝥中提取的3个活性部位YS—Ⅰ、YS—Ⅱ、YS—Ⅲ对人胃癌细胞MGC-803、人食管癌细胞Eca-109和人肝癌细胞HepG2增殖的抑制作用。方法：将从昆虫美洲大蠊、斑蝥中提取的3个活性部位分别作用于MGC-803、Eca-109、HepG2细胞株，采用MTF法检测受试物作用24、48、72小时后细胞增殖抑制情况，计算IC50值，并研究其量效、时效关系。结果：3个由昆虫中提取的活性部位对人源MGC-803、Eca-109、HepG2肿瘤细胞株均有一定的增殖抑制作用，并呈现较好的时间依赖和剂量依赖关系。结论：美洲大蠊提取物YS—Ⅰ及斑蝥不同极性提取物YS—Ⅱ、YS—Ⅲ对人胃癌、食管癌、肝癌细胞具有较好的增殖抑制作用，为今后进一步研发昆虫源低毒、高效的抗肿瘤药物奠定了一定基础。

英文摘要：

Objective To study the proliferation inhibition on MGC -803 cell, Eca - 109 cell and HepG2 cell with three active extracts （YS-I, YS-II, YS- III） from Periplaneta americana and Mylabris. Methods To incubate MGC -803, Eta- 109 and HepG2 cell with three active extracts from Periplaneta americanaand Mylabrisfor 24, 48 and 72 hours, cell proliferation in different concentration and time was determined by MTr assay. Calculating IC50 values and study its dose, time - effect relationship. Results Three active extracts frominsects have a certain proliferation inhibition on MGC - 803 cell, Eca- 109 cell and HepG2 cell. The inhibition effect had good time - dependent and dose - dependent. Conclusion Three active extracts from Periplaneta americanaand Mylabrishave a good proliferation inhibition on MGC - 803, Eta - 109 and HepG2 cell. Laying a foundation for further research and devel- opment of low toxicity, efficient insect anti - tumor drugs.