中文摘要：

目的：利用微小RNA（microR，miRNA）种子序列的反义寡核苷酸研究miR．19a和miR-92a簇在多发性骨髓瘤中的功能及其信号通路分析。方法：实验分为miR-19a种子序列反义寡核苷酸（t-antimiR．19a）组、miR．92a种子序列反义寡核苷酸（t-antimiR-92a）组、随机对照组和空白对照组，通过MTT法检测细胞活力；使用细胞集落形成实验观察集落形成情况；Transwell小室法检测反义寡核苷酸转染细胞的侵袭能力；生物信息学软件miRFo．CU$用来分析miR-19a和miR02a的靶基因和其参与的信号通路。结果：M1Tr结果表明t-antimiR．19a和t-antimiR．92a显著抑制RPMI-8266细胞的生长，t-antimiR-19a和t-antimiR-92a的最佳浓度是0．5μmol／L，最佳作用时间是转染后48h。细胞集落形成实验显示，相比于随机对照组，t-antlmiR-19a组和t-antimiR-92a组的细胞形成集落的能力减弱，集落形成抑制率显著降低。Transwell小室实验显示，相比于随机对照组，t-antimiR-19a组和t-antimiR-92a组的侵袭能力减弱，具有侵袭能力的细胞数显著降低。miRFocus软件分析miR．19a和miR-92a参与多条与肿瘤有关的重要的信号通路。结论：miR-19a和miR-92a有可能作为治疗多发件骨髓瘤的潜在靶点．

英文摘要：

AIM： To study the function of microRNA（ miR）-19 a and miR-92 a by seed-targeting inhibition in multiple myeloma cells and their signal pathways. METHODS： The experiments were divided into t-antimiR-19 a group,tantimiR-92 a group,scramble control group and blank control group. The growth-inhibitory potencies were measured by MTT assay. The ability of cell colony formation was measured by cell colony formation assay. The ability of cell invasion was measured by Transwell experiment. The miR-19 a and miR-92 a target gene signal pathways were integrated by miRFocus software. RESULTS： MTT assay showed that t-antimiR-19 a and t-antimiR-92 a significantly inhibited the viability of multiple myeloma cells,and the best concentration and time were 0. 5 μmol / L and 48 h,respectively. The colony number in t-antimiR-19 a /92 a group was less than that in scramble control group. The transfection with t-antimiR-19 a or t-antimiR-92 a effectively decreased the cell invasion,as the relative invasion cell number was significantly decreased compared with scramble control group. miR-19 a and miR-92 a were involved in m TOR signaling,cell cycle and other cancer pathways.CONCLUSION： miR-19 a and miR-92 a cluster might be a potential target for therapeutic intervention in multiple myeloma.