中文摘要：

目的：探讨mir-483对鼻咽癌细胞增殖、侵袭和迁移能力的影响。方法：利用实时定量聚合酶链反应（RT-q PCR）方法检测不同鼻咽癌细胞株（CNE-1、CNE-2）中mir-483表达量及照射后其表达量的变化;选取鼻咽癌细胞株CNE-1,采用阳离子脂质体法,分别转染mir-483 mimics和阴性对照后,采用MTT细胞增殖实验检测细胞增殖能力;应用划痕实验及transwell实验检测mir-483对鼻咽癌细胞侵袭和迁移能力的影响。结果：mir-483在放疗抵抗鼻咽癌细胞株CNE-1中表达量较放疗敏感鼻咽癌细胞株CNE-2明显增加。过表达mir-483后,其增殖、迁移、侵袭能力均明显增强。结论：mir-483促进鼻咽癌细胞的增殖、侵袭和迁移能力。

英文摘要：

Objective To investigate the effects of mir-483 on the proliferation,invasion and migration ofnasopharyngeal carcinoma cells. Methods RT-q PCR was used to detect mir-483 expression level in differentnasopharyngeal carcinoma cells（CNE-1,CNE-2）and the alteration of mir-483 expression level in these cells afterradiation. CNE-1,one of the NPC cells,was selected. Cationic liposomes transient transfection was used to constructcells which overexpressed mir-483 or negative control. MTT assay was conducted to test the cell proliferationability. Wound scratch assay and transwell migration assay were used to detect the effects of mir-483 on NPCinvasion and migration ability. Results In terms of radiation resistance,the expression level of mir-483 washigher in CNE-1. Overexpressing mir-483 can enhance the proliferation,invasion and migration ability of CNE-1.Conclusions Mir-483 enhances the proliferation,invasion and migration ability of nasopharyngeal carcinoma cells.