中文摘要：

研究生长分化因子-9（growth differentiation factor-9,GDF-9）对牛卵丘细胞增殖的影响。采用MTT法检测不同浓度GDF-9对卵丘细胞增殖的影响,结果表明,GDF-9能促进卵丘细胞的增殖,且GDF-9与卵丘细胞增殖效应存在浓度梯度关系;在卵丘细胞增殖过程中,FSH在一定程度上与GDF-9发挥协同作用。在GDF-9和FSH的作用下,去除卵母细胞的卵丘细胞复合体（oocytectomized cumulus cell complexes,OOX）也可以保持较好的发育形态。实时定量PCR结果表明,随着GDF-9浓度的增加,卵丘细胞扩展相关基因PTX3、HAS2及PTGS2的表达量也增加。总之,以上的研究结果表明,GDF-9可以促进卵丘细胞的增殖,对卵丘细胞功能的发挥起着重要的作用。

英文摘要：

This paper investigated the effect of growth differentiation factor-9 （GDF-9） on the proliferation of bovine cumulus cells. The effect of GDF-9 with different concentration on cumulus cells proliferation was studied by MTT assay. MTT results showed that GDF-9 could promote cumulus cell proliferation by a concentration-dependent manner, and FSH had a synergistic effect with GDF-9 on inducing cumulus cell proliferation. In addition, GDF-9 combined with FSH could promote oocytectomized cumulus complexes （OOX） development. The expression of cumulus cell expansion-related genes PTX3, HAS2 and PTGS2 were examined by Real-time quantitative PCR. The results showed that the expression of expansion-related gene were up-regulated by GDF-9. In conclusion, GDF-9 can promote cumulus cells proliferation and play an important role in regulating function of cumulus cells.