中文摘要：

目的:研究人脐带间质干细胞来源的外切体(hucMSC-Ex)对顺铂诱导的人肾小管上皮HK-2细胞损伤的修复作用,并探讨可能的作用机制。方法:体外建立顺铂诱导的HK-2细胞损伤模型,实验组以16μmol/L浓度的顺铂作用HK-2细胞16 h,再加入hucMSC-Ex共培养,同时选择人肺成纤维细胞的外切体(HFL1-Ex)为对照,8 h后采用蛋白质印迹法和FITC-Tunel技术检测hucMSC-Ex对损伤HK-2细胞的抗凋亡和促增殖情况。未经任何处理的HK-2细胞作为正常对照组,单纯顺铂损伤并加等量PBS为PBS对照组。结果:成功建立了顺铂诱导的HK-2细胞损伤体外模型,蛋白质印迹分析显示hucMSC-Ex处理改善了顺铂对HK-2细胞的损伤,凋亡蛋白Bax表达降低,抗凋亡蛋白Bcl-2表达相应升高。FITC-Tunel检测也显示HK-2细胞的凋亡明显减弱。而MTT分析显示hucMSC-Ex处理后,HK-2细胞的增殖能力显著增强。结论:HucMSC-Ex对顺铂诱导的HK-2细胞损伤有一定的修复效果,可能是通过促进HK-2细胞的增殖和抑制其凋亡来实现的。

英文摘要：

Objective:To investigate the effects of exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs-Ex) on cisplatin induced injury in human renal proximal tubular epithelial (HK-2) cells.Methods:HK-2 cells injury were induced with cisplatin at a concentration of 16 μmol/ L for 16 h.Then injured HK-2 cells were co-cultured with hucMSC-Ex for another 8 h.The apoptosis and proliferation of HK-2 cells were tested by Western blot,and FITC-Tunel assay and MTT respectively.At the same time,healthy HK-2 cells and injured HK-2 cells co-cultured with human lung fibroblasts-derived exosomes (HFL1-Ex) and PBS were used as control.Results:Cisplatin-induced HK-2 injury was confirmed in vitro.Western blot results showed that expression of pro-apoptosis protein Bax was decreased and anti-apoptotic protein Bcl-2 was increased accordingly.FITC-Tunel results also showed HK-2 cells apoptosis was reduced obviously in hucMSCs-Ex group.Furthermore,the MTT assay indicated that hucMSCs-Ex treatment promoted the proliferation of HK-2 cell.Conclusion:hucMSCs-Ex could alleviate cisplatin-induced HK-2 cell injury via promoting cell proliferation and inhibiting apoptosis.