中文摘要：

目的分离自人胃粘膜的乳杆菌抗幽门螺杆菌活性的筛选，为观察乳杆菌与幽门螺杆菌相互作用寻找合适的研究对象。方法从21例胃镜检查者的胃粘膜组织中分离鉴定出6株乳杆菌，检测其在pH2．5～pH4．5和含0．05％～0．2％胆盐条件下的耐受性，并结合琼脂扩散牛津杯法检测其抑制幽门螺杆菌生长的能力及通过细胞试验检测其抗幽门螺杆菌粘附人胃上皮细胞的活性。结果6株乳杆菌分离株中L3产酸量最大，耐酸和高浓度胆盐，其活菌和上清液在血平板中对幽门螺杆菌作用后形成的抑菌环直径均最大，对大肠杆菌的抑菌环大于金黄色葡萄球菌的，处理液相同的两两比较差异有统计学意义（P〈0．05），同时能使幽门螺杆菌粘附人胃上皮细胞的粘附率最低。结论人胃粘膜来源的乳杆菌菌株L3具有较好的耐受性和抗幽门螺杆菌活性，研究结果为幽门螺杆菌感染的微生态治疗提供依据。

英文摘要：

This study aims to screen Lactobacillus strains with Helicobacter pylori antagonistic activity from human gastric mucosa and offer the best research object for the study of the interaction between Lactobacillus and Helicobacter pylori. Six Lactobacillus strains were isolated from the gastric mucosa of 21 people who did gastroscope inspection. The resistant ability of Lactobacillus with different pH （2.5-4.5） and bile salt （0.05%-0.2%） were detected. The research also combined with the determination of their antagonistic potential against Helicobacter pylori by oxford plate assay system and the ability inhibi ted Helicobacter pylori adhere to gastric epithelial cells. Lactobacillus strain L3 had the largest acid production in six strains and could tolerant high acid and high bile salts. The biggest inhibition zones formed when live bacteria and supernatant of strain L3 reacted on Helicobacter pylori, and certain inhibition ability to Escherichia coli and Staphylococcus aureus were found, which were significantly different between any two of the same treatment liquid groups （P〈0.05）. It also made the maximum decrease in Helicobacter pylori adhered to gastric epithelial ceils. Lactobacillus strain L3 isolated from human gastric mucosa had strong antagonistic activity on Helicobacter pylori, This study provides basis for the microcological therapy of Helicobacter pylori related diseases.