中文摘要：

【目的】观察虎杖—桂枝药对（虎桂药对）配伍对尿酸钠诱导的急性痛风性关节炎大鼠Toll样受体4/骨髓样分化因子88（TLR4/My D88）通路的影响,进一步探讨虎桂药对治疗急性痛风的作用机制。【方法】将SD雄性大鼠48只分为正常组、模型组、阴性质粒组、阳性质粒组、虎桂药对（剂量为7 g/kg）＋si RNA组、虎桂药对组（剂量为7 g/kg）,每组各8只。虎桂药对配伍组给予相应药物灌胃,其他各组给予生理盐水灌胃,每日1次,连续10 d。于灌胃第7天模型组和各实验组大鼠均采用踝关节腔内注射尿酸钠复制大鼠急性痛风性关节炎模型,正常组给予同体积的生理盐水;阳性质粒组、虎桂药对﹢si RNA组通过构建好的靶向TLR4基因si RNA（TLR4-si RNA）质粒抑制大鼠体内TLR4基因的表达。观察各组大鼠关节滑膜组织病理形态学变化,采用双抗体夹心法检测外周血炎症因子肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的含量,采用荧光定量PCR和Western blot法检测各组大鼠外周血单核细胞中TLR4、My D88、肿瘤坏死因子受体相关因子6（TRAF-6）m RNA和蛋白表达,免疫组织化学法检测滑膜核转录因子-κB p65（NF-κB p65）的表达。【结果】与正常组比较,模型组滑膜细胞明显增生,内膜下层炎细胞弥漫浸润,以淋巴、单核细胞为主,滑膜表面见大量纤维素粘附。与模型组比较,阳性质粒组,虎桂药对＋si RNA组、虎桂药对组均能明显缓解炎性细胞对滑膜的浸润,改善滑膜细胞增生反应。与正常组比较,模型组血清TNF-α、IL-1β含量显著升高（P〈0.01）,外周血单核细胞TLR4、My D88、TRAF-6 m RNA和蛋白表达,滑膜NF-κB p65表达均显著升高（P〈0.01）;与模型组比较,阳性质粒组,虎桂药对＋si RNA组、虎桂药对组血清中的TNF-α、IL-1β含量显著降低（P〈0.01）,外周血单核细胞TLR4、My D88、TRAF-6 m RNA和蛋白表达以及滑膜NF

英文摘要：

Objective To observe the effects of the drug pair of Rhizoma Polygoni Cuspidati（ Huzhang） and Ramulus Cinnamomi（ Guizhi） on the Toll-like receptor 4 mediated myeloid differentiation factor 88（ TLRs/My D88） signaling pathway of rats with acute gouty arthritis induced by monosodium sodium urate（MSU）, so as to explore its therapeutic mechanism. Methods Forty-eight male SD rats were divided into normal group, modele group, blank plasmid group, positive plasmid group, Huzhang-Guizhi herb-pair（7 g/kg） group, and HuzhangGuizhi herb-pair（7 g/kg） si RNA group, 8 rats in each group. The normal group, plasmid groups and model group were given physiological saline, and the left groups were given the corresponding drug by intragastric administration for 10 continuous days（ once daily）. On the seventh day of intragastric gavage, acute gouty arthritis were induced by injection of MSU into the rat ankle joint, and normal group was injected with the samevolume of normal saline. Positive plasmid group and Huzhang-Guizhi herb-pair si RNA group were injected with the constructed si RNA-TLR4 plasmid targeting TLR4 gene（ TLR4-si RNA） to inhibit the in-vivo TLR4 gene expression. Pathological changes of the synovial tissues were detected, the contents of peripheral blood tumor necrosis factor alpha（ TNF-α） and interleukin 1 beta（ IL-1β） were detected by double antibody sandwich method, and the m RNA and protein expression levels of TLR4, My D88, TNF receptor-associated factor 6（ TRAF-6） in peripheral blood mononuclear cells of rats were detected by real-time fluorescence quantitative polymerase chain reaction（ PCR） and Western blot methods. The nuclear factor kappa B（ NF-κB） p65 immunoactivity was assayed by immunohistochemistry. Results Compared with the normal group, the model group had obvious hyperplasia of synovial cells and the inflammatory cell infiltration（dominated by lymphcytes and monocytes）, and had amount of cellulose adhesive on the synovial membrane surface. Compa