中文摘要：

目的建立一种共振散射（RS）技术测定奈替米星含量的新方法。方法在pH3.78 Britton-Robinson的缓冲液中,奈替米星与丽春红G反应形成离子缔合物,在荧光分光光度计上同步扫描（λex=λem）,体系的共振散射信号增强,并产生新的共振散射光谱,最强的散射峰位于577nm。同时观察了奈替米星与丽春红G反应的适宜条件和对共存物质的影响。结果在波长为577nm处,奈替米星浓度在0.12-10.5μg/ml范围内与RS强度呈良好的线性关系,回归方程为△IRs=7.79c-1.38,相关系数为0.9981,方法的检测限为28.5ng/ml,并用于硫酸奈替米星注射液的测定,回收率在94.70%-106.0%之间。结论该法简便、快速、选择性好、灵敏度高,用于奈替米星含量测定,结果满意。

英文摘要：

Objective To establish a new technigue which is resonance （RS） method detected the contents of netilmicin using ponceau G as probe. Methods In pH 3. 78 Britton-Robinson buffer solution, netilmicin reacts with ponceau G to form as association particles, which enhanced RS signals greatly and produced new RS spectrum, for synchronous scanning yex=yem） by fluorescence spectrophotometer. The strongest RS peak is at 577 nm. The optimum conditions and the effect of co-existing substances on the netilmicirrponceau G system were investigated in details in this paper. Results The RS intensity is directly proportional to the concentration of netilmicin in the range of 0. 12- 10. 5 ug/mL at 577nm. Its regress equation is AIRs = 7. 79c-1. 38, with a relative coefficient of 0. 9 981, with the detection limit 28. 5 ng/ml. The method is applied to the analysis of netilmicin in injection samples. Its recovery rate is in the range of 94.7-106.0%. Conclusion It is a simplicity, and a rapidity, and good selectivity and high sensitive resonance scattering method which is developed for the determination of netilmicin and with satisfactory results.