中文摘要：

目的探讨瘦素（leptin）与阿尔茨海默病发生发展过程的联系。方法全反式维甲酸（all-trans retinoic acid,ATRA）诱导人神经纤维母细胞瘤细胞系SH-SY5Y产生过度磷酸化tau蛋白,模型成功后给予外源性leptin处理,检测磷酸化tau蛋白表达水平。结果模型诱导成功后,PCR检测leptin长型受体ObRb表达上升（P〈0.01）;给予leptin后,Western Blot显示磷酸化tau蛋白表达显著降低（P〈0.01）,细胞形态也随之改变。结论 leptin能够在体外降低阿尔茨海默病相关的过度磷酸化tau蛋白。

英文摘要：

Objective To study the association of leptin with the occurrence and development of Alzheimer＇s disease.Methods A model of tau hyperphosphorylation was induced by all-trans retinoic acid（ATRA） in SH-SY5Y cells and treated with exogenous leptin.Expression of phosphorylated tau protein in SH-SY5Y cells was detected.Results PCR showed that the expression level of leptin long receptor ObRb was higher after the model of hyperphosphorylated tau protein was established（P0.01） whereas western blot showed that the expression level of phosphorylated tau protein was lower and the morphology of SH-SY5Y cells was changed after leptin was given（P0.01）.Conclusion Leptin can reduce Alzheimer＇s disease-related tau protein hyperphosphorylation in vitro.