中文摘要：

The present study provides a comparative understanding of florfenicol deposition kinetics following oral administration at a single dose of 30 mg / kg body weight to Japanese eel( Anguilla japonica) at the temperature of 20℃,24℃ and 28℃,respectively. Approximate 0. 3 m L of blood sample from each eel in these three groups was collected in a row at the time 0. 5,1,2,4,8,12,24 and 36 h after medicated-feed gavage. The concentrations of florfenicol in eel plasma were detected by reversed-phase high performance liquid chromatography( RP- HPLC). The plasma concentration-time data of each eel were analyzed by non-compartmental methods based on statistical moment theory using DAS software. Pharmacokinetics parameters of different groups were tested by one-way analysis of variance using SPSS software. It was found that most of parameters were different significantly among groups( P < 0. 05). In the three groups at 20℃,24℃ and 28℃,pharmacokinetics parameters of peak plasma concentration( Cmax) were( 7. 839 ± 1. 125),( 13. 010 ± 2. 334) and( 18. 267 ± 3. 717) μg/m L,and the time to reach the Cmax( Tmax) were( 6. 500 ± 2. 070),( 4. 500 ± 1. 414) and( 3. 429 ± 0. 926) h,respectively. These suggested that eels at higher temperature absorbed more drug and more quickly. The volumes of distribution( Vz/ F) were( 3. 964 ± 0. 594),( 2. 466 ± 0. 672) and( 1. 841 ± 0. 485) L / kg,respectively.The difference deduced that more florfenicol was bound to tissue in eels at lower temperature. The mean residence time( MRT0- ∞) and the half-life of drug( t1 /2z)in the three respective groups were( 31. 503 ± 7. 117),( 22. 881 ± 4. 940) and( 22. 134 ± 6. 204) h,and( 21. 243 ± 5. 166),( 14. 994 ± 4. 293) and( 14.656 ± 5. 061) h. These parameters showed that the elimination rate of florfenicol in eels at 24℃ or 28℃ was more quickly than that at 20℃. The areas under the concentration- time curve( AUC0- ∞) were( 235. 580 ± 62. 013),( 271. 983 ± 75. 023) and( 353. 192 ± 92. 491) μg·h/m L,respectively. It i

英文摘要：

The present study provides a comparative understanding of florfenicol deposition kinetics following oral administration at a single dose of 30 mg/kg body weight to Japanese eel （ Anguilla japonica） at the temperature of 20℃, 24℃ and 28℃, respectively. Approximate 0.3 mL of blood sample from each eel in these three groups was collected in a row at the time 0.5, 1, 2, 4, 8, 12, 24 and 36 h after medicated-feed gavage. The concentrations of florfenicol in eel plasma were detected by reversed-phase high performance liquid chromatography （ RP - HPLC ）. The plasma concentration-time data of each eel were analyzed by non-compartmental methods based on statistical moment theory using DAS software. Pharmacokinetics parameters of different groups were tested by one-way analysis of variance using SPSS software. It was found that most of parameters were different significantly among groups （ P 〈 0.05 ）. In the three groups at 20℃, 24℃ and 28℃, pharmacokinetics parameters of peak plasma concentration （ Cmax ） were （7. 839 ± 1. 125）, （ 13. 010 ± 2. 334） and （ 18. 267 ± 3. 717 ） μg/mL, and the time to reach the Cmax（Tmax） were （6.500± 2.070）, （4.500 ± 1.414） and （3.429 ± 0.926） h, respectively. These suggested that eels at higher temperature absorbed more drug and more quickly. The volumes of distribution （Vz/F） were （3. 964 ± 0. 594）, （2. 466 ± 0. 672） and （1. 841 ± 0.485） IZkg, respectively. The difference deduced that more florfenicol was bound to tissue in eels at lower temperature. The mean residence time （ MRT0-∞ ） and the half-life of drug （ t1/2, ） in the three respective groups were （31.503 ±7.117）, （22.881 ± 4.940） and （22.134 ± 6.204） h, and （21.243 ±5.166）, （14.994±4.293） and （14. 656± 5. 061 ） h. These parameters showed that the elimination rate of florfenicol in eels at 24℃ or 28℃ was more quickly than that at 20℃. The areas under the concentration -time curve （AUC0-∞ ） were （23