中文摘要：

在那里的背景是为在 osteoarthritic 滑膜炎的渗入的 glycosyltransferases 的一个生物角色的很少报告。这研究的目的是调查表示和 -1,4-galactosyltransferase 的细胞的地点(在一只通过手术导致的老鼠的 -1,4-GaIT-) 膝骨关节炎(OA ) 当模特儿，并且在 OA.Methods 男 Sprague-Dawley 老鼠的致病探索 -1,4-GalT- 的角色随机被划分成三个组：OA 组，假冒的组和正常的组。OA 的模型被前面的十字形的系带办理(ACLT ) 与部分中间的 meniscectomy 在老鼠的右膝建立。从正常老鼠 synovial 织物获得的像成纤维细胞的 synoviocytes (FLS ) 是在与肿瘤坏死因素对待的 synovial 织物，关节的软骨和 FLS 的 -1,4-GalT- mRNA 的 cultured.The 表示 --(TNF-) 是由即时 PCR 的 assayed。西方弄污并且 immunohistochemisty 被用来在蛋白质水平观察 -1,4-GalT- 的表示。染色的两倍 immunofluorescent 被用来在 OA synovium 与像巨噬细胞的 synoviocytes， FLS， neutrophils，和 TNF- 定义 -1,4-GalT- 的地点。在与 lipopolysaccharide ( LPS )和 -1,4-GalT--Ab 被对待的 FLS 的TNF-的改变被连接酶的 immunosorbent 检测-1,4-GalT-的 mRNA 和蛋白质表示在外科以后在二和四个星期与正常、假冒的组相比在 OA 组的 synovial 织物增加了的试金( ELISA ) .Results 然而，没有重要差别出现在关节的软骨。Immunohistochemistry 也显示在在在外科以后的四个星期的 OA synovium 的 -1,4-GalT- 表达式与控制组相比严厉地增加了。与在老鼠 OA 滑膜炎的像巨噬细胞的 synoviocytes， FLSa， neutrophils 和 TNF-co 局部性的 -1,4-GalT- 。而且，在 vitro -1,4-GalT- ，在 FLS 的 mRNA 响应 TNF- 刺激在一个剂量依赖者和时间依赖者举止被影响。当与 antibody.Conclusion -1,4-GalT- 可以玩的反 -1,4-GalT- 对待时， ELISA 表明 TNF- 的表示在 vitro 在 FLS 被稀释在将为在 OA 的进 -1,4-GalT- 的具体机制的进一步的研究提供基础的老鼠 OA synovial 织物的发炎?

英文摘要：

Background There are few reports of a biological role for glycosyltransferases in the infiltration of osteoarthritic synovitis. The aim of this research was to investigate the expression and cellular location of β-1,4-galactosyltransferase Ⅰ （β-1,4-GaIT-Ⅰ） in a surgically-induced rat model of knee osteoarthritis （OA）, and explore the role of β-1,4-GalT-Ⅰ in the pathogenesis of OA.Methods Male Sprague-Dawley rats were randomly divided into three groups： OA group, sham group and normal group. The model of OA was established in the right knees of rats by anterior cruciate ligament transaction （ACLT） with partial medial meniscectomy. Fibroblast-like synoviocytes （FLSs） obtained from normal rat synovial tissue were cultured.The expression of β-1,4-GalT-Ⅰ mRNA in the synovial tissue, articular cartilage and FLSs treated with tumor necrosis factor-α （TNF-α） were assayed by real-time PCR. Western-blotting and immunohistochemisty were used to observe the expression of β-1,4-GalT-Ⅰ at the protein level. Double immunofluorescent staining was used to define the location of the β-1,4-GalT-Ⅰ with macrophage-like synoviocytes, FLSs, neutrophils, and TNF-α in the OA synovium. The alteration of TNF-α in FLSs which were treated with lipopolysaccharide （LPS） and β-1,4-GalT-Ⅰ-Ab were detected by enzyme-linked immunosorbent assay （ELISA）.Results The mRNA and protein expression of β-1,4-GalT-Ⅰ increased in synovial tissue of the OA group compared with the normal and sham groups at two and four weeks after the surgery, however, no significant difference appeared in the articular cartilage. Immunohistochemistry also indicated that the β-1,4-GalT-Ⅰ expression in OA synovium at four weeks after surgery increased sharply compared with the control group. β-1,4-GalT-Ⅰ co-localized with macrophage-like synoviocytes, FLSa, neutrophils and TNF-α in rat OA synovitis. Moreover, in vitro β-1,4-GalT-Ⅰ mRNA in FLSs was affected in a dose- and time-dependent manner in response to TNF