中文摘要：

目的研究颗粒化日本血吸虫Mr22600抗原对小鼠树突状细胞（DCs）功能的影响及诱导CD4^＋CD25^＋调节性T细胞的作用。方法体外实验：分别用Sepharose 4B偶联rSj22.6/26GST抗原与可溶性rSj22.6/26GST抗原负载DCs,流式检测DCs表型变化,混合淋巴细胞增殖实验检测DCs功能。将不同抗原负载的DCs,与CD4^＋T细胞共培养,流式检测观察对CD4^＋CD25^＋Foxp3＋T细胞数量的影响。体内试验：将42只BALB/c小鼠随机分6组（每组6～8只）,A组免疫Sepharose4B偶联rSj22.6/26GST抗原,B组免疫福氏佐剂乳化rSj22.6/26GST抗原,C组免疫rSj22.6/26GST抗原,同时设Sepharose4B对照组（D组）,福氏佐剂对照组（E组）和PBS对照组（F组）,各组均为皮下多点免疫50μg抗原,隔2周加强免疫,共免疫2次。末次免疫后2周处死,无菌取腹股沟淋巴结,制备细胞悬液,流式检测DCs占淋巴结细胞的比例;同时取脾脏,制备成脾脏单个核细胞,流式检测各免疫组CD4^＋CD25^＋Foxp3＋T细胞占脾细胞的比例。用免疫磁珠分选各免疫组CD4^＋CD25^＋T细胞,与CD4^＋CD25-T细胞共培养,氚标记胸腺嘧啶核苷（3H-TdR）掺入法检测细胞增殖情况。结果体外实验结果显示,DCs经可溶性抗原刺激后表面分子CD40、CD80、CD86表达率分别为（43.5±6.2）%、（37.7±0.1）%和（71.4±1.4）%,经Sepharose4B偶联抗原刺激后表达率分别为（31.2±5.4）%、（32.0±1.6）%和（63.8±1.0）%,与可溶性抗原相比,Sepharose4B偶联rSj22.6/26GST抗原不能有效刺激DCs成熟。Sepharose4B偶联rSj22.6/26GST抗原负载DCs可诱导CD4^＋CD25^＋调节性T细胞扩增。体内实验结果显示,Sepharose4B偶联rSj22.6/26GST抗原免疫小鼠可诱导CD4^＋CD25^＋调节性T细胞数量增加,且与可溶性抗原组（cpm值为3558±147）相比,Sepharose4B偶联rSj22.6/26GST抗原组（cpm值为1420±335）来源的CD4^＋CD25^＋调节性T细胞的抑制能力更强。结论 Sepharose4B偶联rSj22.6/26GST抗原与可溶?

英文摘要：

Objective To study the role of Schistosoma japonicum Mr 22 600 particulated-antigen on dendritic cells （DCs） and CD4^＋CD25^＋ regulatory T cells. Methods In in vitro experiments, DCs were pulsed with Sepharose 4B coupling rSj22.6/26GST and soluble rSj22.6/26GST, respectively. The surface molecules of DCs were detected by flow cytometry and the function of DCs was detected by mixed lymphocyte reaction. For the reduction of CD4^＋CD25^＋ T cells, DCs pulsed with Sepharose 4B coupling rSj22.6/26GST and soluble rSj22.6/26GST, respectively, were co-cultured with CD4^＋ T cells isolated from the spleen cells. The percentage of CD4^＋CD25^＋ Foxp3＋ T cells in CD4^＋ T cells was detected by flow cytometry. In in vivo experiments, BALB/c mice were immunized with Sepharose 4B coupling rSj22.6/26GST, Freund′s adjuvant emulsified rSj22.6/26GST, rSj22.6/26GST, Sepharose 4B, Freund′s adjuvant and PBS, respectively. The percentage of DCs in draining lymph nodes and the percentage of CD4^＋CD25^＋Foxp3＋ T cells in spleen cells were detected by flow cytometry. To analyze the inhibitory roles of CD4^＋CD25^＋ T cells on CD4^＋CD25-T cells, CD4^＋CD25^＋ T cells were separated from mice immunized with Sepharose 4B coupling rSj22.6/26GST and Freund′s adjuvant emulsified rSj22.6/26GST, respectively, and co-cultured with CD4^＋CD25-T cells. The proliferation of cells was assessed by [3H] thymidine incorporation method.Results In vitro, the expression rate of the surface molecules of CD40, CD80 and CD86 on the soluble antigen pulsed DCs were （43.5±6.2）%, （37.7±0.1）%, and （71.4±1.4）%, respectively. But on the Sepharose 4B coupling antigen pulsed DCs, they were （31.2±5.4）%, （32.0±1.6）%, and （63.8±1.0）%, respectively, which suggested that the Sepharose 4B coupling rSj22.6/26GST had less stimulating roles on DCs maturation. Furthermore, addition of DCs pulsed with Sepharose 4B coupling rSj22.6/26GST caused the expanding of CD4^＋CD25^＋ T cells. In vivo, immunization of Sepharo