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中文摘要：
研究用芯片毛细管电泳激光诱导荧光检测系统分离测定经7-chloro-4-nitrobenzo-2-oax-1，3-diazole（NBD-Cl）衍生的麻黄碱和伪麻黄碱的实验条件。采用胶束毛细管电动色谱分离体系（12mmol／L SDS＋10mmol／L硼砂缓冲液，pH9．0），在45mm长的通道上实现了麻黄碱和伪麻黄碱的快速分离，一次分离小于1．5min。10—100ms／L范围内，峰高与浓度呈良好的线性关系，麻黄碱、伪麻黄碱的检出限分别是0．83mg／L和1．10mg／L。所建立的方法应用于尿中麻黄碱和伪麻黄碱的分离测定，取得满意的结果。
英文摘要：
A method was developed for the determination of ephedrine （EP） and pseudoephedrine （PEP） dopes in urine with a chip-based capillary electrophoresis and laser induced fluorescence detector system （ CELIF）. The dopes were derived with 7-chloro-4nitrobenzo-2-oax-1,3-diazole （NBD-Cl） to form fluorescent products. By using a separation channel of 45 mm in length, various experimental conditions for CE separation were optimized, and a separation mode of micellar capillary electrokinetic chromatography was adopted. Under the optimized conditions （12 mmol/L sodium dodecyl sulfonate （SDS） ＋ 10 mmol/L tetraborate buffer at pH 9.0, 2600V separation voltage）, EP and PEP can be base-line separated from the reagent components as well as the species existing in the urine within 1.5 min. A linear calibration curve was observed in the concentrations ranging from 10 to 100 mg/L for both EP and PEP, the limits of detection obtained were 0.83 mg/L for EP and 1.10 mg/L for PEP. Satisfactory recoveries were achieved when the developed method was used for determination of EP and PEP concentration spiked into human urine.
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